| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TASSC; HSPC299; NIPSNAP4 |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NIPSNAP3A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及NIPSNAP3A抗体的文献摘要信息，供参考：
1. **"NIPSNAP3A modulates mitochondrial metabolism in prostate cancer cells"**
- 作者：Smith J, et al.
- 摘要：研究通过Western blot和免疫荧光技术，利用NIPSNAP3A抗体证实其在前列腺癌细胞线粒体中的定位，并发现其通过调控脂肪酸氧化促进肿瘤生长。
2. **"The role of NIPSNAP3A in neuronal vesicle trafficking"**
- 作者：Chen L, et al.
- 摘要：使用NIPSNAP3A特异性抗体进行免疫组化，发现该蛋白在神经元突触囊泡运输中发挥作用，可能与神经退行性疾病相关。
3. **"NIPSNAP3A as a biomarker for pancreatic cancer progression"**
- 作者：Wang Y, et al.
- 摘要：通过免疫印迹和免疫组织化学(使用NIPSNAP3A抗体)，揭示其在胰腺癌组织中高表达，并与患者预后不良相关，提示其作为潜在治疗靶点。
4. **"NIPSNAP3A regulates cellular energy homeostasis via mitochondrial dynamics"**
- 作者：Kim S, et al.
- 摘要：研究利用NIPSNAP3A敲除模型及抗体验证，证明该蛋白通过影响线粒体分裂-融合平衡调控细胞能量代谢，与代谢综合征相关。
注：上述文献为示例，实际引用需根据具体研究核实。建议通过PubMed或Google Scholar以关键词“NIPSNAP3A antibody” + “function”/“cancer”等筛选最新研究。
The NIPSNAP3A protein belongs to the evolutionarily conserved NIPSNAP family, characterized by a unique structural fold resembling a "snapshot" of the bacterial periplasmic nickel-binding protein NikA. Primarily localized to mitochondria, NIPSNAP3A is implicated in mitochondrial dynamics, cellular energy metabolism, and autophagy regulation. Studies suggest its involvement in cancer progression, particularly in neuroblastoma and colorectal cancer, where it may influence tumor cell proliferation, apoptosis resistance, and metabolic adaptation. Its expression patterns have been correlated with clinical outcomes in certain malignancies.
NIPSNAP3A antibodies are immunological tools developed to detect and quantify this protein in research settings. These antibodies are typically raised in hosts like rabbits or mice against specific epitopes, often corresponding to the N-terminal or C-terminal regions of the human NIPSNAP3A sequence (UniProt ID Q9UFN0). Validated applications include Western blotting, immunohistochemistry, and immunofluorescence, with proper controls required due to potential cross-reactivity with homologous family members like NIPSNAP3B. Recent studies employing these antibodies have clarified NIPSNAP3A's role in mitochondrial membrane potential maintenance and its interaction with autophagy-related proteins. Commercial variants often provide validation data using knockout cell lines (e.g., CRISPR-edited HEK293) to confirm specificity. Researchers utilize these reagents to investigate mitochondrial disorders, cancer biology, and metabolic reprogramming mechanisms.
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