| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ERA; CEGA; ERA-W; H-ERA; ERAL1A; ERAL1B; HERA-A; HERA-B; PRLTS6 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ERAL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ERAL1抗体的3篇参考文献及其简要摘要：
1. **文献名称**: "ERAL1 is a mitochondrial chaperone involved in the assembly of the 28S small ribosomal subunit"
**作者**: Dennerlein S, et al.
**摘要**: 该研究通过使用ERAL1抗体进行免疫沉淀和蛋白质组学分析，揭示了ERAL1作为线粒体伴侣蛋白在28S核糖体小亚基组装中的关键作用，并发现其缺失导致线粒体翻译缺陷。
2. **文献名称**: "The role of ERAL1 in mitochondrial RNA metabolism and human disease"
**作者**: Metodiev MD, et al.
**摘要**: 文章利用ERAL1特异性抗体进行Western blot和免疫荧光实验，证明ERAL1与线粒体12S rRNA结合，其突变会导致线粒体功能障碍，并与佩尔综合征相关耳聋相关。
3. **文献名称**: "Characterization of ERAL1 as a novel biomarker for hepatocellular carcinoma progression"
**作者**: Li Y, et al.
**摘要**: 通过ERAL1抗体检测肝癌组织样本，发现ERAL1表达水平与肿瘤转移和患者预后显著相关，提示其可能作为肝癌治疗的潜在靶点。
4. **文献名称**: "Structural insights into the GTPase activity of ERAL1 and its interaction with mitochondrial RNA"
**作者**: Zhang X, et al.
**摘要**: 研究结合X射线晶体学和ERAL1抗体的免疫共沉淀实验，解析了ERAL1的GTP酶结构域及其与RNA结合的分子机制，为线粒体疾病机制提供结构基础。
这些文献涵盖了ERAL1在核糖体组装、疾病关联、癌症标志物及结构功能等领域的研究，均涉及ERAL1抗体的实验应用。
ERAL1 (Era-like 1) is a mitochondrial GTPase that functions as a molecular chaperone, primarily involved in the processing and stability of mitochondrial 18S ribosomal RNA (rRNA) and the assembly of the small ribosomal subunit. It plays a critical role in mitochondrial protein synthesis, impacting cellular energy metabolism and overall mitochondrial health. ERAL1 is evolutionarily conserved, sharing homology with bacterial Era proteins, which are essential for ribosome biogenesis.
Antibodies targeting ERAL1 are vital tools for studying its expression, localization, and interactions in mitochondrial biology. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate ERAL1's role in cellular processes, including apoptosis, oxidative stress responses, and mitochondrial dynamics. Dysregulation of ERAL1 has been implicated in diseases such as Perrault syndrome (a genetic disorder affecting hearing and ovarian function) and certain cancers, where mitochondrial dysfunction contributes to pathogenesis. Research using ERAL1 antibodies has also explored its potential as a biomarker or therapeutic target. These studies underscore ERAL1's importance in maintaining mitochondrial integrity and its broader implications in cellular homeostasis and disease mechanisms.
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