| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PECI |
| Uniprot No | O75521 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-394aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMNRTAMRASQKDFENSMNQVKLLKKDPGNE VKLKLYALYKQATEGPCNMPKPGVFDLINKAKWDAWNALGSLPKEAARQN YVDLVSSLSPSLESSSQVEPGTDRKSTGFETLVVTSEDGITKIMFNRPKK KNAINTEMYHEIMRALKAASKDDSIITVLTGNGDYYSSGNDLTNFTDIPP GGVEEKAKNNAVLLREFVGCFIDFPKPLIAVVNGPAVGISVTLLGLFDAV YASDRATFHTPFSHLGQSPEGCSSYTFPKIMSPAKATEMLIFGKKLTAGE ACAQGLVTEVFPDSTFQKEVWTRLKAFAKLPPNALRISKEVIRKREREKL HAVNAEECNVLQGRWLSDECTNAVVNFLSRKSKL |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PECI重组蛋白的参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：*Expression and Functional Characterization of Recombinant PECI in Mammalian Cells*
**作者**：Chen, L., et al.
**摘要**：研究通过哺乳动物表达系统成功制备重组PECI蛋白，验证其在胆固醇代谢中的酶活性，并证明其与脂质转运蛋白的相互作用。
2. **文献名称**：*Optimization of PECI Recombinant Protein Production in Pichia pastoris*
**作者**：Wang, Y., et al.
**摘要**：利用毕赤酵母系统优化PECI重组蛋白的异源表达，通过发酵条件调控使产量提高3倍，并证实其催化长链脂肪酸转化的功能。
3. **文献名称**：*Cryo-EM Structure Determination of Human PECI and Implications for Substrate Binding*
**作者**：Johnson, R., et al.
**摘要**：首次通过冷冻电镜解析人源PECI重组蛋白的高分辨率结构，揭示其底物结合口袋的构象变化机制，为靶向抑制剂设计提供依据。
4. **文献名称**：*PECI Knockout and Recombinant Rescue in a Drosophila Model*
**作者**：Gupta, S., et al.
**摘要**：在果蝇模型中敲除PECI基因导致线粒体功能异常，通过外源性重组PECI蛋白回补实验恢复表型，证实其在能量代谢中的关键作用。
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注：以上文献为模拟内容，实际研究中建议通过学术数据库(如PubMed、Web of Science)以“PECI recombinant protein”或“PECI expression”等关键词检索最新成果。
**Background of PECI Recombinant Protein**
PECI (Prostaglandin E2 Synthase 1), also known as mPGES-1. is a key enzyme in the biosynthesis of prostaglandin E2 (PGE2), a lipid mediator involved in inflammation, pain, fever, and various physiological processes. It catalyzes the conversion of prostaglandin H2 (PGH2) to PGE2. acting downstream of cyclooxygenase (COX) enzymes. PGE2 exerts diverse effects by binding to specific receptors (EP1-EP4), regulating immune responses, vascular tone, and cellular proliferation. Dysregulation of PGE2 signaling is linked to chronic inflammation, cancer, and autoimmune diseases, making mPGES-1 a potential therapeutic target.
Recombinant PECI protein is produced using genetic engineering techniques, where the *PTGES* gene encoding mPGES-1 is cloned into expression systems (e.g., *E. coli*, mammalian cells) for large-scale production. This allows researchers to study the enzyme’s structure, function, and interactions without relying on native tissue sources. Purified recombinant PECI is utilized in biochemical assays, inhibitor screening, and structural studies (e.g., X-ray crystallography) to design drugs that selectively block PGE2 synthesis, aiming to reduce side effects compared to traditional COX inhibitors.
Research on recombinant PECI has advanced understanding of its role in disease mechanisms, particularly in cancer progression and inflammatory disorders. Its overexpression in tumors correlates with poor prognosis, highlighting its relevance in oncology. Additionally, studies using recombinant protein have identified natural and synthetic inhibitors, paving the way for novel anti-inflammatory and anticancer therapies. Overall, recombinant PECI serves as a critical tool for both basic research and drug discovery, bridging insights into prostaglandin biology and clinical applications.
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