| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human HIRIP3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HIRIP3抗体的3篇代表性文献的简要总结(内容基于学术研究主题归纳，非真实文献)：
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1. **文献名称**：*HIRIP3 is a conserved component of the HIRA complex required for chromatin assembly in Drosophila*
**作者**：Smith J, et al.
**摘要**：该研究通过HIRIP3特异性抗体进行免疫沉淀和质谱分析，揭示了HIRIP3在果蝇中与HIRA复合体的相互作用，并证明其在组蛋白H3.3沉积和染色质组装中的关键作用。抗体验证包括Western blot和免疫荧光定位实验。
2. **文献名称**：*Functional characterization of HIRIP3 in mammalian DNA damage response*
**作者**：Chen L, et al.
**摘要**：利用HIRIP3抗体研究其在哺乳动物细胞中的功能，发现HIRIP3通过调控HIRA复合体介导的染色质重塑参与DNA损伤修复。抗体用于Co-IP实验验证与HIRA、ASF1a的相互作用，并通过敲低实验证明其表型依赖性。
3. **文献名称**：*Development and validation of a polyclonal antibody against human HIRIP3 for epigenetic studies*
**作者**：Wang Y, et al.
**摘要**：本研究报道了一种针对人源HIRIP3的多克隆抗体的制备与验证，通过ELISA、Western blot和免疫组化证明其特异性。该抗体被成功应用于检测HIRIP3在多种癌细胞系中的表达差异及核内定位模式。
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**说明**：上述文献为示例性概括，实际研究中建议通过PubMed或Google Scholar以关键词“HIRIP3 antibody”或“HIRA-interacting protein 3”检索最新文献，并关注抗体应用场景(如Co-IP、IF、WB等)及功能机制研究。
The HIRIP3 (HIRA-interacting protein 3) antibody is a tool used to study the HIRIP3 protein, which plays a role in chromatin remodeling and transcriptional regulation. HIRIP3 interacts with HIRA, a histone chaperone complex responsible for depositing the histone variant H3.3 into chromatin, a process critical for DNA repair, gene expression, and developmental programming. This interaction positions HIRIP3 within pathways governing epigenetic modifications and genome stability.
The antibody is primarily utilized in research applications such as Western blotting, immunoprecipitation, and immunofluorescence to detect HIRIP3 expression, localization, and protein-protein interactions. Studies suggest HIRIP3 may contribute to cellular processes like meiosis, DNA damage response, and cell cycle regulation, though its precise molecular mechanisms remain under investigation. Dysregulation of HIRA-associated pathways has been linked to developmental disorders and cancer, implicating HIRIP3 as a potential biomarker or therapeutic target.
Researchers employ HIRIP3 antibodies to explore its functional roles in model organisms and human diseases, particularly in contexts involving chromatin dynamics and epigenetic inheritance. Validating antibody specificity is crucial, as cross-reactivity with related proteins could confound results. Overall, this reagent supports advancing knowledge in epigenetics, genome maintenance, and disease pathogenesis.
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