| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PD2; F23149_1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PAF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PAF1抗体的3篇参考文献示例(文献为模拟虚构，供参考格式)：
1. **文献名称**：*PAF1 Complex Subunit Knockout Reveals Transcriptional Regulation in Stem Cell Maintenance*
**作者**：Smith, A. L., et al.
**摘要**：该研究通过PAF1抗体进行免疫沉淀和ChIP-seq分析，揭示PAF1复合体在维持胚胎干细胞多能性中的关键作用，发现其通过调控RNA聚合酶II的转录延伸影响关键基因(如OCT4)的表达。
2. **文献名称**：*PAF1 Cooperates with Histone Modifiers to Promote Cancer Metastasis*
**作者**：Zhang, Y., et al.
**摘要**：利用PAF1抗体的Western blot和免疫组化实验，作者发现PAF1在结直肠癌中高表达，且与H3K4me3修饰水平正相关，证明PAF1通过表观遗传机制驱动肿瘤转移。
3. **文献名称**：*Development and Validation of a High-Affinity PAF1 Antibody for Functional Studies*
**作者**：Lee, J. H., et al.
**摘要**：该文献描述了一种新型兔源PAF1多克隆抗体的制备与验证，通过免疫荧光和Co-IP实验证实其特异性，并成功应用于探究PAF1在DNA损伤修复中的新功能。
4. **文献名称**：*PAF1 Links Wnt/β-Catenin Signaling to Transcriptional Output in Development*
**作者**：Garcia-Beltran, C., et al.
**摘要**：研究使用PAF1抗体进行果蝇模型中的共定位分析，证明PAF1复合体作为Wnt信号通路下游的调控因子，影响发育相关基因的转录激活。
(注：以上文献为示例，实际引用请通过PubMed或Google Scholar以“PAF1 antibody”及“PAF1 complex”为关键词检索。)
The PAF1 (RNA polymerase II-associated factor 1) antibody is a crucial tool for studying the PAF1 complex, a multifunctional protein assembly conserved from yeast to humans. This complex, comprising subunits like PAF1. LEO1. CTR9. CDC73. and RTF1. plays pivotal roles in transcriptional regulation, histone modification, and chromatin remodeling. It interacts directly with RNA polymerase II (Pol II) to facilitate transcription elongation, mRNA processing, and termination. The PAF1 subunit, in particular, is essential for recruiting histone-modifying enzymes, such as Set1/COMPASS (for H3K4 methylation) and Bre1 (for H2B ubiquitination), thereby influencing epigenetic landscapes.
PAF1 antibodies are widely used in techniques like immunoprecipitation, ChIP-seq, and immunofluorescence to investigate the complex's localization, interactions, and regulatory mechanisms. Dysregulation of the PAF1 complex is implicated in cancers, developmental disorders, and stem cell maintenance defects. For example, PAF1 overexpression correlates with tumor progression in certain cancers, while mutations in associated subunits (e.g., CDC73) link to hyperparathyroidism-jaw tumor syndrome. Researchers also employ PAF1 antibodies to explore its role in cell cycle control, DNA repair, and pluripotency. Validating antibody specificity via knockout controls remains critical, given the complex's structural and functional interdependence with other transcriptional cofactors.
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