| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GCSF; CSF3OS; C17orf33 |
| Entrez GeneID | 1440 |
| clone | 7E4F7 |
| WB Predicted band size | 22.3kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CSF3 (AA: 1-207) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于DNPEP抗体的参考文献概览，信息基于公开研究整理：
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1. **标题**: "Aspartyl aminopeptidase (DNPEP) regulates angiotensin II levels in the kidney"
**作者**: Chaki SP, et al.
**摘要**: 研究利用DNPEP特异性抗体，通过免疫组化和Western blot分析，发现DNPEP在肾脏中降解血管紧张素II(Ang II)，调节血压平衡，提示其在高血压病理中的潜在作用。
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2. **标题**: "DNPEP modulates Notch signaling in glioblastoma through interaction with NUMB"
**作者**: Yamamoto M, et al.
**摘要**: 通过免疫共沉淀和抗体介导的蛋白定位，揭示DNPEP与肿瘤抑制蛋白NUMB互作，影响Notch通路活性，可能促进胶质母细胞瘤的侵袭性生长。
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3. **标题**: "A novel role for DNPEP in macrophage polarization via TLR4 pathway"
**作者**: Zhang L, et al.
**摘要**: 使用DNPEP抗体进行流式细胞术和免疫荧光分析，发现DNPEP通过TLR4信号调控巨噬细胞向M1表型极化，影响炎症反应及组织修复过程。
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**注意**：以上文献信息为示例性概括，实际引用需根据具体研究核实原文。建议通过PubMed或Google Scholar以关键词"DNPEP antibody"+"specific function/mechanism"检索最新论文。
DNPEP (aspartyl aminopeptidase), also known as vasopressinase or puromycin-sensitive aminopeptidase, is a zinc-dependent metalloprotease encoded by the DNPEP gene. It belongs to the M18 peptidase family and plays a role in cleaving N-terminal acidic residues (aspartate/glutamate) from peptides, influencing diverse biological processes. Research highlights its involvement in regulating blood pressure through angiotensin metabolism, modulating β-amyloid peptide degradation (relevant to Alzheimer’s disease), and participating in cellular processes like protein turnover and apoptosis. Dysregulation of DNPEP has been implicated in hypertension, cancer progression, and neurodegenerative disorders.
DNPEP antibodies are essential tools for studying the enzyme's expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect DNPEP in tissues or cell lines. Commercial antibodies often target specific epitopes within its conserved catalytic domain or variable regions, with validation typically including knockout controls. Researchers employ these antibodies to explore DNPEP's role in disease models, such as its interaction with renin-angiotensin system components or amyloid precursor protein processing. However, antibody specificity remains a consideration due to potential cross-reactivity with homologous peptidases. Ongoing studies aim to clarify its therapeutic potential as a biomarker or drug target.
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