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产品描述：兔子补体片断3a(C3a)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C3a含量。C3a 是补体系统激活产生的重要过敏毒素。它参与炎症反应、免疫调节等生理病理过程。研究机制方面，其与 C3a 受体结合，能介导细胞活化、趋化等。在疾病中过度激活可能引发不良免疫反应，相关研究或为疾病治疗提供新靶点。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于兔源实验模型（如败血症、自身免疫疾病或组织损伤模型）中补体系统激活程度的评估，可帮助研究者分析炎症反应机制、药物干预效果或病理状态下C3a的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C3a
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 80-100 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 85-104 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of rabbit C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 84-107 | ||||
| EDTA plasma (n=4) | 96 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.5912.6202.6062.4602001.8891.8741.8821.7361001.3281.3201.3241.178500.7740.7520.7630.617250.4340.4410.4380.29212.50.2960.2900.2930.1476.250.2540.2440.2490.10300.1440.1480.146 | ||||||
货期：3-5 working days
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