| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IEF-SSP-9502 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PWP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PWP1抗体的参考文献示例(内容为假设性概括，仅供参考)：
1. **文献名称**: *PWP1 Mediates Ribosomal RNA Processing via Interaction with WD Repeat Domains*
**作者**: Smith J, et al.
**摘要**: 本研究利用PWP1特异性抗体，通过免疫共沉淀和Western blot技术，揭示了PWP1蛋白在核糖体RNA前体加工中的关键作用，证明其通过与WD重复结构域蛋白的相互作用调控核糖体生物合成。
2. **文献名称**: *PWP1 Deficiency Disrupts Cell Cycle Progression in Human Cells*
**作者**: Lee H, et al.
**摘要**: 通过PWP1抗体进行免疫荧光染色，作者发现PWP1敲低导致细胞周期停滞在G1期，表明其在细胞周期调控中不可或缺，可能与p53信号通路相关。
3. **文献名称**: *PWP1 as a Novel Biomarker in Acute Myeloid Leukemia*
**作者**: García-Ruiz C, et al.
**摘要**: 该研究使用PWP1抗体检测白血病患者样本，发现PWP1高表达与化疗耐药性相关，提示其可能作为AML预后评估的潜在生物标志物。
4. **文献名称**: *Structural Insights into PWP1 Function in Yeast Models*
**作者**: Müller T, et al.
**摘要**: 通过酵母遗传学结合PWP1抗体的ChIP-seq分析，研究揭示了PWP1在维持染色质稳定性及rDNA沉默中的结构基础，为进化保守机制提供证据。
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**注**：以上文献为示例，实际引用需查询PubMed、Web of Science等平台获取真实研究。如需进一步协助定位具体论文，建议提供研究方向或数据库访问权限。
The PWP1 antibody is a research tool designed to detect and study the Periodic Tryptophan Protein 1 (PWP1), a conserved eukaryotic protein belonging to the WD40-repeat family. PWP1 plays a critical role in ribosome biogenesis, particularly in rRNA processing and the assembly of ribosomal subunits. It is part of the UTP (U Three Protein) complex involved in the early stages of 18S rRNA maturation, linking its function to cellular growth, proliferation, and homeostasis. Dysregulation of PWP1 has been implicated in developmental disorders and diseases like cancer, making it a subject of interest in molecular biology and translational research.
The antibody is commonly used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate PWP1 expression, localization, and interactions. Its specificity is validated using knockout controls or siRNA-mediated depletion. Studies employing PWP1 antibodies have shed light on its nucleolar localization, dynamic behavior during the cell cycle, and involvement in ribosome assembly pathways across model organisms, including yeast and mammals. Researchers also utilize it to explore PWP1's role in stress responses, metabolic pathways, and potential crosstalk with oncogenic signaling. As ribosome dysfunction gains attention in disease mechanisms, PWP1 antibodies remain vital for dissecting its biological and pathological relevance.
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