首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠过敏毒素/补体片断4a(C4a)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C4a含量。试剂盒检测范围为39 pg/mL-2500 pg/mL，适用于基础免疫学研究，如炎症性疾病动物模型中补体激活程度的动态监测、药物干预对补体系统影响的评估，或免疫细胞与补体互作机制等实验场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C4a
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：39 pg/mL-2500 pg/mL
灵敏度：9.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse C4a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 96 | ||||
| Range % | 87-105 | |||||
| 1:2000 | Average % | 90 | ||||
| Range % | 84-100 | |||||
| 1:4000 | Average % | 95 | ||||
| Range % | 82-103 | |||||
| 1:8000 | Average % | 96 | ||||
| Range % | 84-103 | |||||
回收率：
| The recovery of mouse C4a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 87-105 | ||||
| EDTA plasma (n=4) | 96 | 82-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 25002.7862.6872.7372.599 12502.1362.0422.0891.951 6251.2531.2651.2591.121 3120.7840.7520.7680.630 1560.5430.5210.5320.394 780.3640.3420.3530.215 390.2420.2390.2410.103 00.1360.1400.138 | ||||||
货期：3-5 working days
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