首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠补体蛋白4(C4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C4B含量。试剂盒检测范围为0.156 μg/mL-10 μg/mL，可应用于小鼠模型中的补体系统功能研究，例如自身免疫性疾病（如红斑狼疮、类风湿关节炎）、炎症反应机制探索或感染性疾病进程中C4蛋白的动态变化分析，亦可用于药物开发中补体相关通路调控效应的科研评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C4b ELISA kit; C4Complement C4-B [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain] ELISA kit
缩写：C4B
Uniprot No.：P01029
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 μg/mL-10 μg/mL
灵敏度：0.039 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:200 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:400 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of mouse C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mOD1OD2AverageCorrected102.6052.6482.6272.46352.3122.3352.3242.1602.51.8571.8671.8621.6981.251.1361.1031.1200.9560.6250.7980.7850.7920.6280.3120.4350.4120.4240.2600.1560.3160.3040.3100.14600.1630.1650.164 | ||||||
货期：3-5 working days
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