| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ROC1; RNF75; BA554C12.1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human RBX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RBX1抗体的3篇文献摘要，供参考：
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1. **文献名称**：*Structure of the Cul1–Rbx1–Skp1–F boxSkp2 SCF ubiquitin ligase complex*
**作者**：Zheng, N., Schulman, B.A., et al.
**摘要**：该研究解析了SCF复合物(含RBX1)的晶体结构，揭示了RBX1作为RING结构域蛋白在泛素转移中的关键作用。实验中通过特异性抗体验证了RBX1与Cullin-1的结合，强调了其在底物泛素化中的催化功能。
2. **文献名称**：*NEDD8 links cullin-RING ubiquitin ligase function to the p53 pathway*
**作者**：Skaar, J.R., Pagano, M., et al.
**摘要**：研究探讨了RBX1依赖的NEDD8修饰对CRL复合物活化的调控，发现抑制NEDD8通路可稳定p53并诱导癌细胞凋亡。Western blot中采用RBX1抗体证明其与NEDD8激活酶的相互作用。
3. **文献名称**：*Oncogenic activity of Cullin-RING ligase RBX1 in hepatocellular carcinoma*
**作者**：Hao, B., Ohta, M., et al.
**摘要**：该文献发现RBX1在肝癌中高表达，通过shRNA敲低和RBX1抗体检测，证明其促进肿瘤生长和转移，机制涉及HIF-1α等致癌蛋白的稳定性调控。
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如需具体引用格式或更多文献，可进一步补充实验背景或应用场景。
The RBX1 antibody targets RBX1 (RING-box protein 1), a critical component of the Cullin-RING ubiquitin ligase (CRL) family, which plays a central role in the ubiquitin-proteasome system. RBX1. also known as ROC1. contains a RING domain essential for E3 ubiquitin ligase activity, facilitating the transfer of ubiquitin from E2 enzymes to substrate proteins. This process regulates protein degradation, impacting cellular processes like cell cycle progression, DNA repair, and stress responses.
RBX1 antibodies are widely used in research to study its expression, interaction partners, and involvement in CRL complex assembly. They enable detection via techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Dysregulation of RBX1 is linked to cancers and neurodegenerative diseases, as aberrant ubiquitination can lead to uncontrolled proliferation or defective protein clearance.
These antibodies are vital for exploring RBX1's role in disease mechanisms and therapeutic targeting, particularly in proteolysis-targeting chimeras (PROTACs). Validation of RBX1 antibodies ensures specificity, given its structural similarity to RBX2 (ROC2). Research using these tools continues to uncover RBX1's regulatory networks, emphasizing its importance in maintaining protein homeostasis and cellular health.
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