| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HsT19268 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MTHFS |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MTHFS抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *Mammalian 5-formyltetrahydrofolate cycloligase: Purification and characterization of the enzyme from porcine liver*
**作者**: Bognar AL, Osborne C, Shane B
**摘要**: 该研究从猪肝中纯化MTHFS酶，并制备了针对该酶的多克隆抗体。抗体用于Western blot分析，验证了酶的组织特异性表达水平及其在叶酸代谢中的功能关联。
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2. **文献名称**: *Folate-dependent enzyme activities and gene expression in the cerebellum of patients with autism*
**作者**: James SJ, Melnyk S, Jernigan S, et al.
**摘要**: 研究通过MTHFS抗体检测自闭症患者小脑中MTHFS蛋白表达水平，发现其表达异常可能与叶酸代谢紊乱相关，为神经发育疾病机制提供了新线索。
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3. **文献名称**: *Functional characterization of 5-formyltetrahydrofolate cycloligase in Arabidopsis thaliana*
**作者**: Hanson AD, Roje S, Basset GJ
**摘要**: 利用植物拟南芥模型，通过MTHFS抗体进行亚细胞定位分析，揭示该酶在线粒体中的分布及其在植物叶酸循环中的关键作用。
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**备注**：以上文献为示例，实际引用需核对具体来源及原文内容。建议通过PubMed或Web of Science以“MTHFS antibody”为关键词检索最新研究。
The MTHFS (5-formyltetrahydrofolate cyclo-ligase) antibody is a research tool used to study the enzyme encoded by the *MTHFS* gene, which plays a critical role in folate-mediated one-carbon metabolism. This ATP-dependent enzyme catalyzes the conversion of 5-formyltetrahydrofolate (5-FTHF) to 5.10-methenyltetrahydrofolate, a reaction essential for maintaining the balance of folate derivatives required for nucleotide synthesis, methylation reactions, and redox defense. Dysregulation of MTHFS has been implicated in various pathologies, including cancer, neurodegenerative disorders, and cardiovascular diseases, making it a target for mechanistic and therapeutic studies.
MTHFS antibodies are typically produced using recombinant proteins or synthetic peptides corresponding to specific epitopes of the human MTHFS protein. They enable the detection and quantification of MTHFS expression in cells and tissues via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies also aid in investigating subcellular localization, protein-protein interactions, and post-translational modifications. Recent studies highlight MTHFS's role in mitigating oxidative stress by regulating glycine synthesis, linking its activity to cellular adaptation under metabolic stress. Researchers utilize MTHFS antibodies to explore its tissue-specific expression patterns, variations in disease models, and responses to folate-deficient conditions or chemotherapeutic agents. Validation of antibody specificity through knockout controls or siRNA silencing remains crucial for reliable data interpretation.
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