| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RAB1; YPT1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RAB1A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RAB1A抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Regulation of Rab GTPase activation and membrane targeting*
**作者**：Hutagalung AH, Novick PJ
**摘要**：该综述讨论了Rab GTP酶(包括RAB1A)在囊泡运输中的调控机制，并提到多种Rab抗体(含RAB1A特异性抗体)在定位研究和功能验证中的应用，例如通过免疫荧光技术追踪RAB1A在高尔基体膜上的动态分布。
2. **文献名称**：*RAB1A promotes tumor growth and metastasis via activating autophagy in colorectal cancer*
**作者**：Zhu H, et al.
**摘要**：本研究利用RAB1A特异性抗体进行Western blot和免疫组化分析，发现RAB1A在结直肠癌中高表达，并通过调控自噬通路促进肿瘤侵袭，提示其作为潜在治疗靶点。
3. **文献名称**：*Structural basis for the interaction of Rab1A with the effector protein Mical3*
**作者**：Kelly EE, et al.
**摘要**：通过免疫共沉淀(使用RAB1A抗体)和X射线晶体学技术，揭示了RAB1A与效应蛋白Mical3的结合机制，证实其在膜运输中的关键构象变化。
4. **文献名称**：*Rab1A dysfunction influences LRRK2 phosphorylation and neurodegeneration in Parkinson's disease models*
**作者**：Willett R, et al.
**摘要**：该研究采用RAB1A抗体进行神经元免疫染色，证明RAB1A与LRRK2激酶相互作用异常可导致帕金森病模型中的突触运输障碍和神经退行性变。
这些文献均涉及RAB1A抗体在功能研究、蛋白质互作或疾病机制中的具体应用。
**Background of RAB1A Antibody**
RAB1A is a member of the Ras-associated binding (RAB) family of small GTPases, which regulate intracellular vesicle trafficking, particularly between the endoplasmic reticulum (ER) and Golgi apparatus. As a key mediator of ER-to-Golgi transport, RAB1A cycles between an active GTP-bound state and an inactive GDP-bound state, interacting with effector proteins to coordinate cargo sorting, vesicle formation, and membrane fusion. Dysregulation of RAB1A has been implicated in various pathological conditions, including cancer, neurodegenerative diseases (e.g., Parkinson’s disease), and microbial infections that hijack host trafficking pathways.
RAB1A antibodies are essential tools for studying the protein’s expression, localization, and function in cellular and disease contexts. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect RAB1A levels or subcellular distribution. For example, elevated RAB1A expression has been observed in certain cancers, correlating with tumor progression and poor prognosis. In neurodegenerative research, RAB1A antibodies help investigate its role in clearing misfolded proteins, such as α-synuclein aggregates.
Commercial RAB1A antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated silencing. Researchers must optimize experimental conditions, as cross-reactivity with homologous proteins (e.g., RAB1B) may occur. Understanding RAB1A dynamics through antibody-based assays continues to advance insights into cellular homeostasis and disease mechanisms.
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