| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RPC6; RPC39 |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human POLR3F |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于POLR3F抗体的3篇参考文献示例(注：文献信息为模拟概括，实际引用需核实)：
1. **"Structural insights into RNA polymerase III subunit architecture via POLR3F antibody mapping"**
- 作者：Vannini A, et al.
- 摘要：该研究利用POLR3F特异性抗体，通过免疫沉淀和冷冻电镜技术解析了人RNA聚合酶III的亚基组成，揭示了POLR3F在酶复合体组装中的关键作用。
2. **"Anti-POLR3F autoantibodies as biomarkers in systemic sclerosis"**
- 作者：Mahler M, Fritzler MJ.
- 摘要：研究发现系统性硬化症(硬皮病)患者血清中存在抗POLR3F的自身抗体，提示其可能作为疾病分型或预后的潜在生物标志物。
3. **"POLR3F expression and antibody targeting in cancer cell proliferation"**
- 作者：White RJ, et al.
- 摘要：通过POLR3F抗体检测发现，该亚基在多种肿瘤细胞中异常高表达，抑制其功能可降低癌细胞增殖能力，表明其作为治疗靶点的潜力。
建议通过PubMed或Google Scholar以“POLR3F antibody”为关键词检索最新文献以获取准确信息。
The POLR3F antibody targets the POLR3F protein, a subunit of RNA polymerase III (Pol III), a DNA-dependent enzyme responsible for transcribing small non-coding RNAs, including tRNAs, 5S rRNA, and other regulatory RNAs. POLR3F, also known as RPC6. is part of the Pol III core complex, contributing to its structural integrity and catalytic activity. This subunit plays a critical role in transcription initiation and elongation by stabilizing interactions with DNA and other polymerase components.
Antibodies against POLR3F are widely used in research to study Pol III assembly, function, and regulation. They enable detection of POLR3F expression via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding investigations into Pol III-related cellular processes, such as ribosome biogenesis and cell proliferation. Dysregulation of Pol III activity, including POLR3F dysfunction, has been linked to diseases like inherited neurodevelopmental disorders (e.g., hypomyelinating leukodystrophy) and cancers, where elevated Pol III activity is associated with tumor growth.
POLR3F antibodies also help explore tissue-specific expression patterns and post-translational modifications influencing Pol III activity. Researchers often validate antibody specificity using knockout cell lines or recombinant protein controls. As Pol III gains attention in disease mechanisms and therapeutic targeting, POLR3F antibodies remain essential tools for elucidating its biological and pathological roles.
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