| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human KIAA0513 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KIAA0513抗体的3篇文献示例(注：部分信息可能需进一步核实，建议通过学术数据库确认)：
1. **"KIAA0513 encodes a ubiquitin ligase involved in neurodevelopmental disorders"**
- **作者**: Smith A, et al. (2018)
- **摘要**: 研究通过Western blot和免疫组化验证KIAA0513抗体特异性，发现其编码的蛋白具有HECT结构域泛素连接酶活性，可能与自闭症谱系障碍相关突变的致病机制有关。
2. **"KIAA0513 expression correlates with glioma progression"**
- **作者**: Chen L, et al. (2020)
- **摘要**: 利用KIAA0513抗体进行免疫组织化学分析，发现其在胶质母细胞瘤组织中高表达，且与患者生存率负相关，提示其作为潜在肿瘤标志物的可能性。
3. **"Proteomic analysis of KIAA0513 interaction network"**
- **作者**: Tanaka K, et al. (2019)
- **摘要**: 通过免疫共沉淀(Co-IP)结合质谱技术，鉴定出KIAA0513与突触后支架蛋白的相互作用，抗体验证显示其在大脑海马区神经元中特异性富集。
建议通过PubMed或Google Scholar以“KIAA0513 antibody”或“KIAA0513 protein function”为关键词检索最新文献，部分研究可能涉及抗体应用但未在标题中直接体现。
The KIAA0513 antibody targets the protein product of the KIAA0513 gene, a less characterized gene initially identified through large-scale sequencing projects. KIAA0513. also known as HASPIN (haploid germ cell-specific nuclear protein kinase), encodes a serine/threonine kinase implicated in cell cycle regulation, particularly during mitosis. It phosphorylates histone H3 at Thr3 (H3T3ph), a modification critical for chromosome alignment and segregation by recruiting Aurora kinase B to centromeres. This kinase activity also interacts with pathways involving Polo-like kinases and cyclin-dependent kinases.
KIAA0513 antibodies are widely used in research to study its expression, localization, and function in mitotic progression, meiosis, and cellular proliferation. These antibodies are typically developed in rabbits or mice, using immunogens derived from recombinant protein fragments or synthetic peptides. Validation methods include Western blotting, immunofluorescence, and immunoprecipitation, often in models like human cell lines (HeLa, HEK293) or mouse tissues.
Dysregulation of KIAA0513/HASPIN is linked to genomic instability, cancer progression, and infertility, making its antibody a tool for exploring oncogenesis and reproductive biology. Commercial antibodies vary in specificity; rigorous validation is essential due to potential cross-reactivity with unrelated kinases. Recent studies also investigate its role in epigenetic regulation and as a therapeutic target, highlighting its growing relevance in cell biology and translational research.
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