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产品描述：牛胱天蛋白酶7(Casp-7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Casp7含量。Casp7（Caspase-7）是一种参与细胞凋亡过程的半胱氨酸型内肽酶，属于caspase家族。它在细胞凋亡的执行阶段发挥关键作用，通过切割下游底物如PARP来促进程序性细胞死亡。Casp7的表达和活性与多种疾病如癌症、阿尔茨海默病等密切相关。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品可用于科研领域细胞凋亡机制研究、药物或毒素诱导的凋亡效应评估、免疫相关疾病模型分析等场景，例如探究病原感染、肿瘤治疗或神经系统疾病中的Casp - 7表达变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Casp7
Uniprot No.：F1MD58
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine Casp-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 85-101 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 88-102 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 83-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-106 | |||||
回收率：
| The recovery of bovine Casp-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-112 | ||||
| EDTA plasma (n=4) | 93 | 84-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.5462.4572.5022.369201.8831.9051.8941.761101.1361.1241.1300.99750.7260.7110.7190.5862.50.4070.3970.4020.2691.250.3050.3130.3090.1760.6250.1960.2040.2000.06700.1300.1360.133 | ||||||
货期：3-5 working days
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