首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胱天蛋白酶3(Casp-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CASP3含量。CASP3是一种由CASP3基因编码的酶，属于胱天蛋白酶家族，参与细胞凋亡过程。其研究机制涉及CASP3的激活、底物特异性、结构、激活途径、抑制和与其他蛋白质的相互作用。CASP3在细胞凋亡中起着关键作用，负责染色质浓缩和DNA碎裂。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本试剂盒通过特异性捕获目标蛋白并基于酶标信号放大系统实现精准定量，适用于探索凋亡相关机制、评估药物干预效果或疾病模型中Casp - 3的动态变化等科研用途。为体外研究细胞凋亡通路提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Casp3 ELISA Kit; Cpp32Caspase-3 ELISA Kit; CASP-3 ELISA Kit; EC 3.4.22.56 ELISA Kit; Apopain ELISA Kit; Cysteine protease CPP32 ELISA Kit; CPP-32 ELISA Kit; LICE ELISA Kit; Protein Yama ELISA Kit; SREBP cleavage activity 1 ELISA Kit; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ELISA Kit
缩写：CASP3
Uniprot No.：P70677
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse caspase 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 98-107 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 84-95 | |||||
回收率：
| The recovery of mouse caspase 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-105 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.6372.5522.5952.437 102.1752.1062.1411.983 51.4641.5241.4941.336 2.50.8320.8290.8310.673 1.250.5430.5240.5340.376 0.6250.3660.3390.3530.195 0.3120.2690.2850.2770.119 00.1590.1570.158 | ||||||
货期：3-5 working days
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