| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EIF2B; EIF-2Bbeta |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human EIF2B2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EIF2B2抗体的3篇文献摘要示例(注：部分文献信息为模拟概括，实际引用时需核实原文)：
1. **文献名称**：*Mutations in EIF2B2 linked to vanishing white matter disease impair translational regulation*
**作者**：van der Knaap, M.S., et al.
**摘要**：研究通过Western blot和免疫组化分析，利用EIF2B2特异性抗体发现，EIF2B2基因突变导致其蛋白稳定性下降，影响真核起始因子复合物的功能，进而引发脑白质病中应激反应的异常调控。
2. **文献名称**：*Characterization of EIF2B subunits in cellular stress response using isoform-specific antibodies*
**作者**：Pavitt, G.D., et al.
**摘要**：该文献开发了针对EIF2B各亚基(包括EIF2B2)的特异性抗体，并通过免疫沉淀实验证明EIF2B2在整合应激条件下与其他亚基的动态相互作用，揭示其在调控全局蛋白质合成中的关键作用。
3. **文献名称**：*Antibody-based profiling of EIF2B complex in neurodegenerative disorders*
**作者**：Fogli, A., et al.
**摘要**：研究利用EIF2B2抗体对患者脑组织样本进行分析，发现EIF2B复合物表达水平与神经系统退行性病变的严重程度相关，提示其作为潜在生物标志物的可能性。
(注：以上文献为领域内典型研究方向示例，实际文献需通过PubMed或学术数据库检索确认。)
The EIF2B2 antibody targets the eukaryotic translation initiation factor 2B subunit β (EIF2Bβ), a critical component of the EIF2B complex. This heteropentameric complex (α, β, γ, δ, ε subunits) regulates the guanine nucleotide exchange activity of eukaryotic initiation factor 2 (EIF2), a key step in initiating protein synthesis under stress conditions. EIF2Bβ (encoded by the *EIF2B2* gene) contributes to the structural stability of the complex and modulates its catalytic function.
EIF2B2 antibodies are primarily used in research to study cellular stress responses, translation regulation, and neurological disorders. Mutations in *EIF2B2* are linked to vanishing white matter disease (VWMD), a leukodystrophy characterized by myelin loss and stress-induced neurological deterioration. These antibodies enable detection of EIF2B2 expression levels, subcellular localization (e.g., cytoplasmic distribution), and post-translational modifications in disease models or patient samples.
As a research tool, EIF2B2 antibodies are applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Their specificity helps elucidate EIF2B complex dynamics during integrated stress response (ISR) activation, where phosphorylation of EIF2α modulates global translation. Understanding EIF2B2 dysfunction provides insights into VWMD pathogenesis and potential therapeutic strategies targeting translational regulation pathways.
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