Rabbit Polyclonal TRUB1 Antibody

The TRUB1 antibody is a tool used to detect TRUB1 (TruB Pseudouridine Synthase Family Member 1), a conserved eukaryotic enzyme involved in RNA modification. TRUB1 catalyzes the isomerization of uridine to pseudouridine (Ψ) at specific positions in ribosomal RNA (rRNA) and small nuclear RNA (snRNA), a process critical for RNA stability, translation fidelity, and cellular stress responses. Structurally, TRUB1 contains an N-terminal catalytic domain with methyltransferase activity and a C-terminal PUA domain for RNA binding. Dysregulation of TRUB1 has been linked to diseases, including cancer and neurodegenerative disorders, highlighting its role in cell proliferation and genome integrity. Researchers use TRUB1 antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study its expression, localization, and interactions. Commercial TRUB1 antibodies are typically validated for specificity using knockout controls or siRNA knockdown. However, variability in cross-reactivity or batch consistency requires careful optimization. Recent studies also explore TRUB1's involvement in viral RNA processing and its potential as a therapeutic target. Its dual enzymatic and RNA-binding functions make it a key subject in epitranscriptomics research.