| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Adrenodoxin; ADX; FDX; fdx1; Ferredoxin 1; Hepatoredoxin; LOH11CR1D;;Ferredoxin 1 |
| WB Predicted band size | Calculated MW: 19 kDa ; Observed MW: 13 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Ferredoxin 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ZFYVE27抗体的3篇参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**: "ZFYVE27 regulates endosomal trafficking and its mutations lead to hereditary spastic paraplegia"
**作者**: Mannan AU et al.
**摘要**: 本研究通过免疫印迹和免疫荧光技术，利用特异性ZFYVE27抗体证实其在神经元中的内体定位，并揭示其突变导致细胞内运输缺陷，与遗传性痉挛性截瘫(HSP)发病相关。
2. **文献名称**: "Characterization of a polyclonal antibody against the C-terminal domain of ZFYVE27/protrudin"
**作者**: Hashimoto Y et al.
**摘要**: 文章报道了一种针对ZFYVE27 C端结构域的多克隆抗体的制备与验证，证明其可用于检测人脑组织及细胞系中ZFYVE27的表达，并发现其在神经突生长中的潜在作用。
3. **文献名称**: "ZFYVE27 interacts with microtubule-associated proteins and modulates axonal transport"
**作者**: Saita S et al.
**摘要**: 通过免疫共沉淀(Co-IP)结合ZFYVE27抗体，研究发现ZFYVE27与动力蛋白复合物相互作用，调控轴突运输功能，为神经退行性疾病机制提供新见解。
注：以上文献为示例，实际研究中建议通过PubMed或Google Scholar以“ZFYVE27 antibody”“protrudin antibody”等关键词检索真实文献。
The ZFYVE27 antibody targets the protein encoded by the ZFYVE27 gene, also known as protrudin. This gene belongs to the FYVE-domain protein family, characterized by zinc finger motifs that bind phosphatidylinositol 3-phosphate (PI3P), facilitating membrane trafficking and intracellular signaling. Protrudin plays a critical role in regulating endosomal trafficking, neurite outgrowth, and endoplasmic reticulum (ER)-endosome contact sites, essential for neuronal development and function. It interacts with motor proteins (e.g., kinesin) and membrane fusion machinery to mediate vesicle transport and organelle dynamics.
ZFYVE27 antibodies are essential tools for detecting protrudin expression and localization in research. They are typically produced using recombinant protein fragments or synthetic peptides as immunogens in host species like rabbits or mice. Specificity is validated via knockout cell lines or siRNA-mediated knockdown. Applications include Western blotting, immunofluorescence, and immunohistochemistry to study protrudin’s role in cellular processes, such as autophagy, axonal elongation, and ER membrane remodeling.
Mutations in ZFYVE27 are linked to hereditary spastic paraplegia (HSP), a neurodegenerative disorder. Research using these antibodies has elucidated protrudin’s involvement in HSP pathogenesis and its potential role in Alzheimer’s disease. Their utility extends to exploring membrane trafficking mechanisms and therapeutic targets for neurological conditions.
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