| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IFRX; IRXL1; C10orf48 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MKX |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MKX抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *Mohawk is a transcription factor that promotes meniscus cell phenotype and tissue repair and reduces osteoarthritis development*
**作者**: Sun H, et al.
**摘要**: 本研究利用MKX抗体通过免疫组化分析MKX蛋白在小鼠半月板组织中的表达，证实MKX通过调控细胞外基质基因维持半月板细胞表型，并证明其过表达可促进损伤修复，延缓骨关节炎进展。
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2. **文献名称**: *The transcription factor Mohawk controls differentiation and fibrosis in murine tendinopathy*
**作者**: Ito Y, et al.
**摘要**: 研究通过Western blot和免疫荧光结合MKX抗体，发现MKX在肌腱细胞分化中起关键作用。基因敲除小鼠模型显示MKX缺失导致肌腱纤维化加剧，提示其作为治疗肌腱病变的潜在靶点。
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3. **文献名称**: *Mohawk promotes the maintenance of peripheral nerve structure and function through direct regulation of myelin protein genes*
**作者**: Liu H, et al.
**摘要**: 利用MKX抗体进行染色质免疫沉淀(ChIP)实验，证明MKX直接调控髓鞘蛋白基因表达。在周围神经损伤模型中，MKX缺失导致髓鞘结构异常和神经传导功能受损。
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注：以上文献名为虚构示例，实际文献需通过学术数据库检索确认。
The MKX antibody is a crucial tool in biomedical research, specifically targeting the Mohawk Homeobox (MKX) transcription factor. MKX, a member of the three-amino-acid loop extension (TALE) homeodomain protein family, plays a pivotal role in regulating tissue development, particularly in tendons, muscles, and cartilage. Discovered in 2010. MKX is essential for tendon differentiation and maintenance by modulating extracellular matrix synthesis and cell proliferation. Its dysfunction is linked to musculoskeletal disorders, including tendon pathologies and osteoarthritis.
Researchers employ MKX antibodies to detect and quantify MKX protein expression in various experimental models. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study MKX's spatial-temporal expression during embryogenesis and tissue repair. Commercial MKX antibodies are typically raised in rabbits or mice, with validation in knockout controls to ensure specificity.
Recent studies highlight MKX's role beyond tendon biology, including its involvement in chondrogenesis and muscle fiber organization. MKX antibodies have also facilitated investigations into regenerative medicine, particularly in stem cell differentiation toward tendon-like lineages. Additionally, they aid in exploring MKX's interactions with signaling pathways like TGF-β and BMP, offering insights into therapeutic targets for connective tissue diseases. Proper validation remains critical due to potential cross-reactivity with other homeodomain proteins.
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