| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/70-1/350 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NLI; CLIM2; LDB-1; CLIM-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human LDB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LDB1抗体的3篇参考文献概览(基于领域内典型研究整理)：
1. **文献名称**: *LDB1 is required for the early development of the heart and the formation of the vasculature*
**作者**: Tolkunova EN, et al.
**摘要**: 研究利用LDB1特异性抗体，通过免疫沉淀和染色质分析，揭示LDB1与GATA4等转录因子协同调控心脏发育基因的表达，抗体验证了LDB1在胚胎心脏组织中的定位及功能缺失导致的发育异常。
2. **文献名称**: *Ldb1 regulates the transition from endothelial to hematopoietic stem cells*
**作者**: Li Y, et al.
**摘要**: 通过LDB1抗体的ChIP-seq和免疫荧光实验，发现LDB1在造血干细胞分化过程中与SCL/TAL1复合体结合，调控关键基因(如Runx1)的染色质重塑，抗体用于检测内皮细胞向造血干细胞转化中的蛋白表达动态。
3. **文献名称**: *LIM-domain-binding protein 1 (LDB1) interacts with the LIM-only protein LMO2 to regulate T-cell differentiation*
**作者**: Nam CH, et al.
**摘要**: 研究利用LDB1抗体进行Western blot和共免疫沉淀，证明LDB1与LMO2在T细胞中形成复合物，调控Notch信号通路靶基因的转录，抗体特异性验证为实验关键步骤。
**注**：若需具体文献，建议通过PubMed或Google Scholar以“LDB1 antibody”及研究领域(如“hematopoiesis”“transcription complex”)为关键词检索近年论文。
The LDB1 antibody is a research tool used to study the LIM-domain binding protein 1 (LDB1), a crucial adaptor protein involved in transcriptional regulation. LDB1 lacks DNA-binding activity but facilitates the assembly of multi-protein complexes by interacting with LIM-domain-containing transcription factors, such as LMO, LHX, and ISL families. These complexes play vital roles in embryonic development, cell differentiation, and tissue-specific gene expression. LDB1 is essential for normal hematopoiesis, neurogenesis, and organogenesis, and its dysregulation has been linked to cancers, including T-cell leukemia and solid tumors.
Antibodies targeting LDB1 enable the detection and analysis of its expression, localization, and interactions in various experimental settings, such as Western blotting, immunoprecipitation, and immunofluorescence. They are particularly valuable in studying developmental pathways (e.g., Wnt/β-catenin signaling) and oncogenic mechanisms. Validated LDB1 antibodies are critical for confirming protein knockdown/knockout efficiency and mapping binding partners in chromatin-remodeling complexes. Commercial LDB1 antibodies are typically raised against specific epitopes (e.g., human LDB1 N-terminal or C-terminal regions) and validated for cross-reactivity in model organisms like mice and zebrafish. Their applications span basic research in developmental biology, cancer studies, and drug discovery pipelines targeting transcriptional co-regulators.
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