首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胆囊收缩素/肠促胰酶肽(CCK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCK含量。CCK即缩胆囊素，是一种广泛存在于胃肠道和中枢神经系统的脑肠肽。它在调节消化、食物摄取等方面有重要作用。研究机制上，主要通过与CCK受体结合启动细胞内信号转导，影响神经和内分泌系统功能，在胃肠病和神经疾病领域研究较多。试剂盒检测范围为31.25-2000 pg/mL，为研究消化系统功能、神经调节机制以及肥胖或代谢相关疾病的动物模型提供可靠工具，适用于基础科研中探索CCK在胃肠动力、能量代谢或脑肠轴调控等领域的分子机制，亦可应用于药物开发或营养干预实验中的生物标志物分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CckCholecystokinin ELISA Kit; CCK) [Cleaved into: Cholecystokinin-33 ELISA Kit; CCK33); Cholecystokinin-12 ELISA Kit; CCK12); Cholecystokinin-8 ELISA Kit; CCK8)] ELISA Kit
缩写：CCK
Uniprot No.：P09240
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL - 2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 105 | |
| Range % | 100-112 | ||
| 1:2 | Average % | 91 | |
| Range % | 85-97 | ||
| 1:4 | Average % | 90 | |
| Range % | 85-94 | ||
| 1:8 | Average % | 93 | |
| Range % | 89-97 |
回收率：
| The recovery of mouse CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.5732.4632.5182.394 10001.9561.8251.8911.767 5001.3021.2451.2741.150 2500.8040.8410.8230.699 1250.5370.5120.5250.401 62.50.3200.3010.3110.187 31.250.2250.2340.2300.106 00.1210.1270.124 | ||||||
货期：3-5 working days
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