首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL2含量。CCL2是一种趋化因子，在炎症反应、免疫调节和肿瘤进展等方面发挥关键作用。其研究机制主要围绕它与受体结合，激活下游信号通路，招募免疫细胞到特定组织部位。深入研究CCL2对理解相关疾病发病机制和开发靶向治疗有重要意义。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研人员可通过定量分析不同实验条件下（如炎症模型、药物干预或基因修饰）大鼠样本中MCP-1的动态变化，为探究其在免疫调控机制、心血管疾病或神经退行性疾病中的作用提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccl2 ELISA Kit; Je ELISA Kit; Mcp1 ELISA Kit; Scya2 ELISA Kit; C-C motif chemokine 2 ELISA Kit; Immediate-early serum-responsive protein JE ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
缩写：CCL2
Uniprot No.：P14844
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 95 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.4592.4452.4522.289 10001.9241.7861.8551.692 5001.2621.2511.2571.094 2500.7670.7550.7610.598 1250.4730.4680.4710.308 62.50.3340.3270.3310.168 31.250.2460.2380.2420.079 00.1660.1600.163 | ||||||
货期：3-5 working days
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