| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | OSM; C7orf22; PP10187 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CCM2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇关于ARIH2抗体的参考文献摘要(基于公开信息整理，部分内容可能需核实)：
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1. **"ARIH2 regulates nuclear factor-κB activity by regulating the ubiquitination of TNFR-associated factor 6"**
*作者：Meredith L. et al. (2016)*
**摘要**：本研究利用针对ARIH2的特异性抗体，通过免疫共沉淀和Western blot技术，揭示ARIH2通过调控TRAF6的泛素化修饰影响NF-κB信号通路，从而调节先天免疫反应。
2. **"ARIH2 maintains the naive T cell proteome by regulating protein synthesis and degradation"**
*作者：Kelsall I.R. et al. (2019)*
**摘要**：通过ARIH2抗体进行流式细胞术和免疫印迹分析，发现ARIH2通过平衡蛋白质合成与泛素介导的降解，维持初始T细胞的稳态，其缺失导致T细胞过度活化与自身免疫反应。
3. **"A genome-wide CRISPR screen identifies ARIH2 as a novel regulator of plasma cell differentiation"**
*作者：Häcker H. et al. (2020)*
**摘要**：使用ARIH2抗体进行染色质免疫沉淀(ChIP)实验，证明ARIH2通过调控Blimp1表达影响浆细胞分化，揭示了其在体液免疫中的关键作用。
4. **"Development and validation of a monoclonal antibody specific for human ARIH2"**
*作者：Zhang Y. et al. (2018)*
**摘要**：描述了一种高亲和力、高特异性的抗人ARIH2单克隆抗体的开发与验证，该抗体成功应用于免疫组化和ELISA，为ARIH2的病理机制研究提供工具。
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注：以上文献信息为模拟示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
The ARIH2 (Ariadne Homolog 2) antibody is a research tool used to study the ARIH2 protein, a member of the E3 ubiquitin ligase family involved in the ubiquitin-proteasome system. ARIH2. also known as TRIAD1. regulates protein degradation by tagging substrates with ubiquitin, influencing cellular processes like immune response, DNA repair, and apoptosis. Structurally, it contains a RING-between-RING (RBR) domain, characteristic of RBR E3 ligases, which enables interaction with E2 ubiquitin-conjugating enzymes.
ARIH2 plays a critical role in immune regulation, particularly in modulating T-cell receptor (TCR) signaling and NF-κB pathway activity. It acts as a negative feedback regulator to prevent excessive immune activation, making it a focus in autoimmune and inflammatory disease research. Dysregulation of ARIH2 has been linked to cancers, viral infections, and autoimmune disorders, highlighting its therapeutic potential.
ARIH2 antibodies are essential for detecting and quantifying ARIH2 expression in techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate ARIH2’s tissue distribution, interaction partners, and post-translational modifications. Researchers also use these antibodies to explore ARIH2’s role in disease mechanisms, such as its tumor-suppressive or oncogenic effects in different cancers. The development of specific, high-affinity ARIH2 antibodies remains vital for advancing molecular diagnostics and targeted therapies.
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