| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/70-1/350 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MRD36; PP2AA; PR65A; PP2AAALPHA; PP2A-Aalpha |
| WB Predicted band size | 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PPP2R1A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPP2R1A抗体的3篇代表性文献及其摘要内容：
1. **文献名称**: "The role of PPP2R1A in phosphorylation-dependent regulation of Wnt signaling in cancer"
**作者**: Sangodkar J, et al.
**摘要**: 该研究通过免疫沉淀和Western blot分析，发现PPP2R1A作为PP2A全酶的支架亚基，通过调控β-catenin磷酸化状态影响Wnt信号通路。研究在结直肠癌细胞中利用特异性抗体证实了PPP2R1A缺失导致PP2A复合体功能异常，促进肿瘤生长。
2. **文献名称**: "PPP2R1A mutations drive cisplatin resistance in ovarian cancer through loss of PP2A-mediated apoptosis regulation"
**作者**: Cheng Y, et al.
**摘要**: 研究通过免疫组化和Western blot技术，发现卵巢癌中PPP2R1A高频突变导致PP2A酶活性降低。使用特异性抗体验证突变体蛋白的稳定性下降，揭示了其对化疗药物抵抗的分子机制。
3. **文献名称**: "Epigenetic silencing of PPP2R1A mediates histone modification alterations in lung adenocarcinoma"
**作者**: Kleiman LB, et al.
**摘要**: 该研究利用ChIP-seq和免疫荧光技术，结合PPP2R1A特异性抗体，发现肺癌中PPP2R1A启动子甲基化导致其表达沉默，进而引起组蛋白修饰异常和癌基因激活，为表观遗传治疗提供了新靶点。
注：以上为模拟文献，实际研究中建议通过PubMed等数据库以"PPP2R1A antibody"+"specific species (如human)"+"application method (如Western blot)"为关键词检索最新文献。真实研究多集中于PPP2R1A在癌症突变(如子宫内膜癌、小细胞肺癌)中的功能验证。
The PPP2R1A antibody targets the protein phosphatase 2 regulatory subunit A alpha (PPP2R1A), a critical component of the protein phosphatase 2A (PP2A) complex. PP2A is a serine/threonine phosphatase involved in regulating diverse cellular processes, including cell cycle progression, apoptosis, and signal transduction. The PPP2R1A-encoded Aα subunit serves as a structural scaffold for the PP2A holoenzyme, facilitating interactions between the catalytic (C) and regulatory (B) subunits. This assembly determines substrate specificity and phosphatase activity.
PPP2R1A antibodies are widely used in research to study PP2A complex composition, localization, and function in both normal and pathological conditions. Mutations or dysregulation of PPP2R1A have been implicated in cancer, neurodegenerative diseases, and developmental disorders. For example, somatic PPP2R1A mutations are observed in endometrial, lung, and ovarian cancers, often disrupting PP2A-mediated tumor-suppressive functions. Antibodies against PPP2R1A enable detection of expression levels, post-translational modifications (e.g., methylation, phosphorylation), and interactions with viral oncoproteins that hijack PP2A signaling.
These antibodies are essential tools in techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation. Their applications extend to exploring therapeutic strategies targeting PP2A dysregulation, emphasizing PPP2R1A's role as a potential biomarker or drug target.
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