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Mouse C-C motif chemokine 28(CCL28) ELISA kit

  • 中文名: 小鼠C-C趋化因子28(CCL28)ELISA kit
货号: IPD-EL004792MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠C-C趋化因子28(CCL28)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CCL28含量。CCL28,又称粘膜相关上皮趋化因子(MEC),是一种CC趋化因子,存在于几乎所有粘膜组织中,具有免疫刺激作用。CCL28能与CCR3和CCR10结合,介导免疫反应、病毒感染、癌症及抗菌作用。研究显示,CCL28-CCR10通路在类风湿关节炎中促进单核细胞迁移,可能参与滑膜炎症和骨破坏。试剂盒检测范围为78 pg/ml- 5000 pg/ml,灵敏度为19.5 pg/ml。可广泛用于探究感染性疾病、自身免疫反应或黏膜屏障功能异常的小鼠模型中CCL28的动态变化,为黏膜免疫调控机制、炎症通路分析及药物干预效果评估提供可靠工具;尤其在肠道和呼吸道相关疾病机制研究中具有重要价值。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Ccl28 ELISA Kit; Scya28 ELISA Kit; C-C motif chemokine 28 ELISA Kit; Small-inducible cytokine A28 ELISA Kit
  • 缩写:
    CCL28
  • Uniprot No.:
    Q9JIL2
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    78 pg/ml - 5000 pg/ml
  • 灵敏度:
    19.5 pg/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CCL28 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    97


    Range %

    92-105


    1:2

    Average %

    92


    Range %

    88-95


    1:4

    Average %

    86


    Range %

    80-94


    1:8

    Average %

    89


    Range %

    84-98


  • 回收率:

    The recovery of mouse CCL28 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    87-98


    EDTA plasma (n=4)

    102

    95-108


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    5000

    2.692

    2.921

    2.807

    2.681


    2500

    2.157

    2.166

    2.162

    2.036


    1250

    1.469

    1.522

    1.496

    1.370


    625

    0.866

    0.848

    0.857

    0.731


    312

    0.523

    0.545

    0.534

    0.408


    156

    0.324

    0.336

    0.330

    0.204


    78

    0.233

    0.252

    0.243

    0.117


    0

    0.129

    0.122

    0.126




  • 货期:
    3-5 working days

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