Mouse C-C motif chemokine 25(CCL25) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL004789MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠C-C趋化因子25（CCL25）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL25含量。CCL25，又称胸腺表达趋化因子TECK，是一种CC趋化因子家族的小细胞因子，主要表达于胸腺和肠上皮。CCL25通过与其受体CCR9结合，参与T细胞的发育和细胞迁移，以及多种炎症性疾病，并促进炎症反应。其相互作用还可通过Akt/蛋白激酶B激活抑制细胞凋亡，支持T细胞存活。试剂盒检测范围为78 pg/ml- 5000 pg/ml，灵敏度为19.5 pg/ml。适用于多种科研场景，例如探索肠道炎症性疾病模型（如结肠炎）中趋化因子的表达调控机制、评估免疫细胞迁移相关信号通路的功能研究，或用于药物开发过程中靶向CCL25/CCR9轴的治疗性化合物体外效果验证。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Ccl25 ELISA Kit; Scya25 ELISA Kit; Teck ELISA Kit; C-C motif chemokine 25 ELISA Kit; Chemokine TECK ELISA Kit; Small-inducible cytokine A25 ELISA Kit; Thymus-expressed chemokine ELISA Kit
缩写：
CCL25
Uniprot No.：
O35903
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/ml - 5000 pg/ml
灵敏度：
19.5 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CCL25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	101
	Range %	98-105
1:2	Average %	99
	Range %	96-103
1:4	Average %	89
	Range %	85-94
1:8	Average %	97
	Range %	92-103

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	2.357	2.366	2.362	2.231
2500	1.825	1.812	1.819	1.688
1250	1.260	1.366	1.313	1.182
625	0.751	0.745	0.748	0.617
312	0.478	0.498	0.488	0.357
156	0.313	0.322	0.318	0.187
78	0.217	0.221	0.219	0.088
0	0.128	0.133	0.131