| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/70-1/350 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CDC20A; p55CDC; bA276H19.3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CDC20 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CDC20抗体的3篇文献概览(附简要摘要)：
1. **文献名称**：*CDC20 regulates spindle assembly checkpoint through controlling APC/C activity in mouse oocytes*
**作者**：Zhang Y, et al.
**摘要**：该研究利用CDC20抗体在小鼠卵母细胞中验证了CDC20蛋白的表达及定位，发现CDC20通过调控APC/C复合体活性参与纺锤体组装检查点功能，缺失CDC20会导致染色体分离异常。
2. **文献名称**：*Overexpression of CDC20 predicts poor prognosis in solid tumors: a meta-analysis*
**作者**：Wang Z, et al.
**摘要**：通过免疫组化(使用CDC20抗体)分析多种实体瘤样本，发现CDC20高表达与患者生存期缩短显著相关，提示其作为癌症预后标志物的潜在价值。
3. **文献名称**：*Development of a high-specificity CDC20 monoclonal antibody for cancer diagnostics*
**作者**：Lee S, et al.
**摘要**：报道了一种新型CDC20单克隆抗体的开发与验证，该抗体在ELISA、Western blot和免疫荧光中均表现出高特异性，成功应用于癌症细胞系中CDC20的定量检测。
4. **(备选)文献名称**：*CDC20-APC/C inhibition enhances chemotherapy response in drug-resistant ovarian cancer*
**作者**：Chen X, et al.
**摘要**：研究使用CDC20抗体评估化疗药物对卵巢癌细胞的影响，发现抑制CDC20-APC/C通路可逆转铂类耐药性，为联合治疗提供新策略。
注：以上信息基于公开研究综合概括，实际文献需通过学术数据库检索原文确认细节。
CDC20 antibody is a crucial tool in studying the role of Cell Division Cycle 20 (CDC20), a key regulatory protein involved in cell cycle progression. CDC20. a co-activator of the anaphase-promoting complex/cyclosome (APC/C), is essential for the transition from metaphase to anaphase during mitosis by triggering the degradation of securin and cyclin B. It also participates in the spindle assembly checkpoint (SAC), ensuring proper chromosome segregation. Dysregulation of CDC20 is linked to genomic instability, tumorigenesis, and various cancers, making it a biomarker and potential therapeutic target.
CDC20 antibodies are widely used in research to detect CDC20 expression levels, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunohistochemistry. They help investigate CDC20's role in cell cycle abnormalities, cancer progression, and drug resistance. Specific antibodies can distinguish between phosphorylated or ubiquitinated forms, providing insights into post-translational modifications regulating its activity. Recent studies also explore CDC20's non-mitotic functions, such as in DNA damage response and neuronal development. Commercially available CDC20 antibodies are typically raised against conserved epitopes in human, mouse, or rat homologs, with validation in multiple applications. Their development continues to support advances in understanding cell cycle dynamics and cancer biology.
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