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Mouse Macrophage Inflammatory Protein 1α,MIP-1α ELISA kit

  • 中文名： 小鼠巨噬细胞炎性蛋白1α(MIP-1α/CCL3)ELISA Kit
货号: IPD-E04663m
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠巨噬细胞炎性蛋白1α(MIP-1α/CCL3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL3含量。CCL3属于趋化因子，在免疫系统中发挥重要作用，可招募免疫细胞到炎症或感染部位。研究机制方面，其能与特定受体结合，激活下游信号通路，调控免疫细胞的迁移、活化等过程，在炎症、肿瘤等疾病研究中是重要靶点。试剂盒检测范围为7.8 pg/ml- 500 pg/ml，灵敏度为1.95 pg/ml。该产品适用于体外实验研究，如炎症模型构建、免疫应答动态监测、药物干预效果评估等科研场景，其高灵敏度和宽检测范围可满足低丰度样本的检测需求，为实验室研究炎症相关信号通路及免疫调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Ccl3 ELISA kit; Mip1a ELISA kit; Scya3C-C motif chemokine 3 ELISA kit; Heparin-binding chemotaxis protein ELISA kit; L2G25B ELISA kit; Macrophage inflammatory protein 1-alpha ELISA kit; MIP-1-alpha ELISA kit; SIS-alpha ELISA kit; Small-inducible cytokine A3 ELISA kit; TY-5 ELISA kit
  • 缩写：
    CCL3
  • Uniprot No.：
    P10855
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/ml - 500 pg/ml
  • 灵敏度：
    1.95 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    90-100


    1:2

    Average %

    93


    Range %

    88-97


    1:4

    Average %

    101


    Range %

    97-110


    1:8

    Average %

    84


    Range %

    80-88


  • 回收率：

    The recovery of mouse MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    97

    92-103


    EDTA plasma (n=4)

    90

    85-95


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    500

    2.520

    2.352

    2.436

    2.260


    250

    2.056

    2.176

    2.116

    1.940


    125

    1.503

    1.604

    1.554

    1.378


    62.5

    0.839

    0.875

    0.857

    0.681


    31.2

    0.524

    0.535

    0.530

    0.354


    15.6

    0.349

    0.336

    0.343

    0.167


    7.8

    0.252

    0.239

    0.246

    0.070


    0

    0.171

    0.180

    0.176




  • 货期：
    3-5 working days

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