| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/80-1/400 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PP7; PPEF; PPP7C; PPP7CA |
| WB Predicted band size | 76 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human PPEF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPEF1抗体的虚构参考文献示例(实际文献请通过学术数据库查询)：
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1. **文献名称**: *PPEF1 regulates photoreceptor development in Drosophila via calcium-dependent signaling*
**作者**: Thompson R, et al.
**摘要**: 本研究利用抗PPEF1抗体进行免疫荧光染色，揭示了PPEF1在果蝇视网膜光感受器细胞中的特异性表达模式，并发现其通过调控钙离子信号通路参与光感受器的分化与存活。
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2. **文献名称**: *Characterization of PPEF1 in mammalian retinal degeneration models*
**作者**: Chen L, et al.
**摘要**: 通过Western blot和免疫组化技术(使用PPEF1抗体)，作者发现PPEF1蛋白在小鼠视网膜变性模型中表达显著下调，提示其可能与视杆细胞的功能维持及退行性病变相关。
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3. **文献名称**: *PPEF1 interacts with rhodopsin kinase in vertebrate photoreceptors*
**作者**: Gupta S, et al.
**摘要**: 本研究通过免疫共沉淀(使用PPEF1抗体)和质谱分析，首次报道了PPEF1与视紫红质激酶(Rhodopsin Kinase)的相互作用，表明其在视觉信号转导中可能发挥调节作用。
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**注意事项**：以上文献为模拟示例，实际研究中PPEF1抗体的应用可能集中在神经发育、癌症或代谢疾病领域。建议通过PubMed或Google Scholar搜索关键词“PPEF1 antibody”或“PPEF1 protein phosphatase”获取真实文献。
PPEF1 (Protein Phosphatase with EF-Hand Domain 1), also known as PPEF, is a serine/threonine phosphatase belonging to the PPP family. It is encoded by the *PPEF1* gene in humans and is characterized by a unique N-terminal EF-hand calcium-binding domain, distinguishing it from other phosphatases. PPEF1 is predominantly expressed in sensory neurons and retinal tissues, suggesting a role in visual signal transduction and neuronal development. Studies link it to phototransduction pathways, where it may dephosphorylate key components to regulate light adaptation.
PPEF1 antibodies are immunological tools developed to detect and study the expression, localization, and function of PPEF1 in biological systems. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate PPEF1's involvement in diseases, including retinal degeneration and neurological disorders. Research also explores its potential tumor-suppressive roles in certain cancers, though mechanisms remain under investigation.
Commercial PPEF1 antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides as immunogens. Validation includes testing for specificity via knockout controls or siRNA knockdown. Despite progress, challenges persist in understanding PPEF1's full interactome and substrate profile, driving ongoing demand for reliable antibodies to advance functional and clinical studies.
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