首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠单核细胞趋化蛋白2(MCP-2/CCL8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL8含量。CCL8是一个靶点。它属于趋化因子家族，在炎症反应、免疫调节等生理过程中发挥作用。研究机制方面，其可与相应受体结合，介导免疫细胞的迁移和活化，在肿瘤、自身免疫病等疾病进展中可能扮演重要角色，是潜在治疗靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，本试剂盒通过高特异性抗体对实现精准捕获和信号放大，适用于小鼠模型中的炎症机制研究、免疫调控实验或相关疾病（如自身免疫病、感染性疾病）的分子标志物分析，能够稳定检测生物样本中低至皮克级别的目标蛋白含量，为科研人员探索趋化因子网络功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccl8 ELISA Kit; Mcp2 ELISA Kit; Scya8C-C motif chemokine 8 ELISA Kit; Monocyte chemoattractant protein 2 ELISA Kit; Monocyte chemotactic protein 2 ELISA Kit; MCP-2 ELISA Kit; Small-inducible cytokine A8 ELISA Kit
缩写：CCL8
Uniprot No.：Q9Z121
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 85-88 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 96-101 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 89-95 | |||||
回收率：
| The recovery of mouse MCP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 84-92 | ||||
| EDTA plasma (n=4) | 95 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.9552.7812.8682.7202002.1382.1562.1471.9991001.4451.5321.4891.341500.9210.9030.9120.764250.5840.5610.5730.42512.50.3330.3240.3290.1816.250.2410.2530.2470.09900.1420.1530.148 | ||||||
货期：3-5 working days
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