| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SIII; TCEB1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ELOC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ELOC抗体的参考文献示例(注：以下内容为模拟示例，非真实文献，实际引用时请核实来源)：
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1. **文献名称**：*Interaction of VHL Tumor Suppressor with Elongin C: Functional Insights from Antibody-Based Assays*
**作者**：Duan DR, et al.
**摘要**：研究利用ELOC特异性抗体通过免疫共沉淀和Western blot技术，揭示了VHL蛋白与Elongin C(ELOC)的直接结合机制，阐明了其在转录调控复合体中的结构基础。
2. **文献名称**：*Elongin C Modulates HIF-1α Degradation in Hypoxia Signaling*
**作者**：Maxwell PH, et al.
**摘要**：通过ELOC抗体的免疫沉淀实验，证明ELOC作为VHL复合体核心组分，介导缺氧诱导因子HIF-1α的泛素化降解，为靶向癌症代谢通路提供依据。
3. **文献名称**：*ELOC Overexpression in Renal Cell Carcinoma: Prognostic Implications*
**作者**：Li Y, et al.
**摘要**：采用ELOC抗体的免疫组化分析，发现ELOC在肾透明细胞癌中异常高表达，且与患者生存率显著相关，提示其作为潜在诊断标志物。
4. **文献名称**：*Structural Dynamics of the Elongin Complex Revealed by Cryo-EM*
**作者**：Stebbins CE, et al.
**摘要**：结合ELOC抗体的Western blot验证，利用冷冻电镜解析Elongin复合体的三维结构，阐明其与RNA聚合酶II相互作用的分子机制。
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建议通过PubMed或Google Scholar以关键词“ELOC antibody”、“TCEB1”、“Elongin C”检索最新文献，并关注其在癌症、转录调控或泛素化通路中的研究。
The ELOC antibody targets the Elongin C (ELOC) protein, a critical component of the Elongin complex, which plays multifaceted roles in transcriptional regulation and protein ubiquitination. ELOC, also known as TCEB1. forms a heterotrimeric complex with Elongin A (ELOA) and Elongin B (ELOB) to enhance RNA polymerase II transcriptional elongation by suppressing transient pausing. Additionally, ELOC interacts with the von Hippel-Lindau (VHL) tumor suppressor protein as part of the VHL-Elongin B-Elongin C (VBC) complex, which functions as a substrate-recognition module in the Cullin 2 (CUL2)-based E3 ubiquitin ligase. This ligase targets hypoxia-inducible factor (HIF) for proteasomal degradation under normoxic conditions, a key regulatory mechanism in oxygen sensing and tumor suppression.
ELOC antibodies are essential tools for studying its dual roles in transcription and ubiquitination. They are used to investigate ELOC expression, complex formation, and dysregulation in diseases like renal cell carcinoma (linked to VHL mutations) or other cancers where HIF signaling is aberrant. Researchers employ these antibodies in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to explore ELOC’s involvement in cellular responses to hypoxia, DNA repair, and tumorigenesis. The development of specific ELOC antibodies has advanced understanding of its structural interactions within the Elongin and VBC complexes, as well as its broader implications in gene expression and protein stability pathways.
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