首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠程序性细胞死亡配体1(CD274)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD274含量。CD274，即PD-L1，在肿瘤免疫逃逸中扮演重要角色。其背景在于它可与T细胞表面PD-1结合，抑制T细胞活性。研究机制围绕阻断二者结合展开，以恢复T细胞对肿瘤细胞的免疫杀伤功能，目前基于此的免疫治疗研究是热点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于肿瘤免疫微环境研究、免疫治疗药物评估或炎症性疾病机制探索等方向本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cd274 ELISA Kit; B7h1 ELISA Kit; Pdcd1l1 ELISA Kit; Pdcd1lg1 ELISA Kit; Pdl1Programmed cell death 1 ligand 1 ELISA Kit; PD-L1 ELISA Kit; PDCD1 ligand 1 ELISA Kit; Programmed death ligand 1 ELISA Kit; B7 homolog 1 ELISA Kit; B7-H1 ELISA Kit; CD antigen CD274 ELISA Kit
缩写：CD274
Uniprot No.：Q9EP73
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD274 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-97 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of mouse CD274 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-99 | ||||
| EDTA plasma (n=4) | 99 | 94-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.8052.6502.7282.560 10002.4042.3752.3902.222 5001.7091.8101.7601.592 2500.9941.0081.0010.833 1250.5950.5470.5710.403 62.50.3720.3550.3640.196 31.250.2570.2550.2560.088 00.1710.1640.168 | ||||||
货期：3-5 working days
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