WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/100-1/200 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
Aliases | YANK1 |
WB Predicted band size | 46 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | Fusion protein of human STK32A |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于STK32A抗体的示例文献(注:部分信息为假设性示例,实际文献需通过学术数据库验证):
1. **文献名称**:*"Generation and Application of a Polyclonal Antibody for Detecting Human STK32A in Cellular Models"*
**作者**:Zhang L, et al.
**摘要**:本研究开发了一种针对人源STK32A的多克隆抗体,验证了其在Western blot和免疫荧光中的特异性,并发现STK32A在HEK293细胞中高表达,可能与细胞周期调控相关。
2. **文献名称**:*"STK32A Antibody-Based Profiling Reveals Its Role in Neuronal Differentiation"*
**作者**:Kumar S, et al.
**摘要**:通过特异性抗体检测,发现STK32A在小鼠胚胎干细胞向神经元分化过程中表达上调,敲低实验表明其通过调控MAPK通路影响分化进程。
3. **文献名称**:*"A Monoclonal Antibody Targeting STK32A Inhibits Tumor Growth in Pancreatic Cancer Models"*
**作者**:Tanaka M, et al.
**摘要**:研制了一种靶向STK32A的单克隆抗体,体内实验显示其能显著抑制胰腺癌移植瘤的生长,提示STK32A可能作为癌症治疗的新靶点。
4. **文献名称**:*"STK32A Interaction Network Identified by Co-Immunoprecipitation Using a Novel Antibody"*
**作者**:Gomez P, et al.
**摘要**:利用新开发的STK32A抗体进行免疫共沉淀,鉴定出多个互作蛋白(如PKC家族成员),揭示了其在细胞骨架重塑中的潜在功能。
建议通过PubMed或Google Scholar以关键词“STK32A antibody”或“STK32A kinase”检索最新文献,或查阅抗体生产商(如Abcam、CST)的技术手册引用文献。
The STK32A (Serine/Threonine Kinase 32A) antibody is a tool used to study the STK32A protein, a member of the CAMK (calcium/calmodulin-dependent protein kinase) family. STK32A, also known as YANK1 or TESK2. is a poorly characterized kinase implicated in cellular processes like cytoskeletal organization, signal transduction, and possibly neuronal development. Its exact physiological role remains unclear, but it shares structural homology with other kinases involved in regulating cell motility and differentiation.
The STK32A antibody is typically developed in hosts like rabbits or mice using immunogenic peptides derived from conserved regions of the protein. It enables detection of STK32A in techniques such as Western blotting, immunohistochemistry, or immunofluorescence. Researchers utilize it to explore STK32A's expression patterns, subcellular localization, and interactions in tissues like the brain, testes, or cancer cells. Specificity is validated via knockout controls or siRNA silencing. Despite limited functional data, STK32A's dysregulation has been tentatively linked to neurological disorders and cancer progression, driving interest in its molecular mechanisms. The antibody thus serves as a critical reagent for elucidating STK32A's biological significance.
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