| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NCE2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human UBE2F |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于UBE2F抗体的参考文献示例(注：以下为模拟内容，实际文献需根据具体数据库检索验证)：
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1. **文献名称**：*UBE2F-Specific Antibody Development and Application in Detecting NEDDylation Activity*
**作者**：Smith J, et al.
**摘要**：本研究开发了一种高特异性的UBE2F抗体，用于检测细胞中UBE2F介导的NEDDylation修饰。通过Western blot和免疫荧光验证了抗体在多种癌细胞系中的有效性，并揭示了UBE2F在调控Cullin蛋白功能中的作用。
2. **文献名称**：*UBE2F Antibody Reveals Its Role in Lung Cancer Proliferation via CRL5 Complex Regulation*
**作者**：Li X, et al.
**摘要**：利用UBE2F特异性抗体，研究者发现UBE2F通过激活CRL5泛素连接酶复合体促进肺癌细胞增殖。抗体在免疫共沉淀实验中证实了UBE2F与CRL5的相互作用，为靶向治疗提供了依据。
3. **文献名称**：*A Novel Monoclonal Antibody for UBE2F: Validation in Clinical Tumor Samples*
**作者**：Garcia R, et al.
**摘要**：报道了一种新型UBE2F单克隆抗体的开发，通过免疫组化分析显示UBE2F在结直肠癌组织中高表达，且与患者预后不良相关，提示其作为生物标志物的潜力。
4. **文献名称**：*UBE2F-Knockdown and Antibody-Based Functional Studies in Neurodegeneration Models*
**作者**：Kim S, et al.
**摘要**：通过UBE2F抗体阻断实验，研究发现UBE2F缺失可减少异常蛋白聚集，延缓神经退行性疾病模型中的病理进程，表明其在蛋白质质量控制中的关键作用。
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建议通过PubMed、Web of Science等平台以关键词“UBE2F antibody”、“UBE2F function”进一步检索确认具体文献。
UBE2F (Ubiquitin-Conjugating Enzyme E2 F) is a member of the E2 ubiquitin-conjugating enzyme family, which plays a critical role in the ubiquitin-proteasome system (UPS). This system regulates protein degradation, cellular homeostasis, and various signaling pathways by tagging target proteins with ubiquitin for proteasomal destruction. UBE2F specifically partners with E3 ligases, such as cullin-RING ligases (CRLs) like CUL5-RBX2 complexes, to facilitate the transfer of ubiquitin to substrate proteins. Unlike other E2 enzymes, UBE2F is unique in its ability to catalyze the neddylation of cullin proteins, a post-translational modification essential for CRL activation and substrate recognition.
Research has highlighted UBE2F's involvement in diverse biological processes, including viral infection responses, cell cycle regulation, and stress signaling. For example, it contributes to the degradation of viral proteins and cellular regulators like p53 under certain conditions. Dysregulation of UBE2F has been linked to diseases such as cancer, where its overexpression may promote tumorigenesis by destabilizing tumor suppressors or stabilizing oncoproteins.
UBE2F antibodies are essential tools for studying its expression, localization, and interactions in cellular contexts. These antibodies enable detection via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Validated antibodies help elucidate UBE2F's role in UPS mechanisms, its pathological implications, and potential therapeutic targeting. Ongoing studies aim to clarify its substrate specificity and regulatory networks, positioning UBE2F as a molecule of interest in both basic research and drug development.
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