首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人CD4分子(CD4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CD4含量。人CD4分子是T淋巴细胞表面的辅助受体，与MHC II类分子结合，辅助T细胞识别抗原。CD4在HIV感染中起关键作用，作为病毒的主要受体，其数量减少与疾病进展密切相关。研究CD4分子有助于了解免疫调节和HIV感染机制。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品可为免疫疾病机制研究、病毒感染与宿主免疫互作分析、免疫治疗药物开发等科研领域提供数据支持，尤其适用于HIV相关研究、自身免疫性疾病模型评估或免疫细胞功能实验中CD4表达的动态监测，为探索免疫调控网络提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD antigen CD4
缩写：CD4
Uniprot No.：P01730
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of Human CD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 83-95 | ||||
| EDTA plasma (n=4) | 96 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.4852.5842.5352.362201.9282.0271.9781.805101.3651.2651.3151.14250.8620.8420.8520.6792.50.5640.5770.5710.3981.250.3790.3650.3720.1990.6250.2710.2630.2670.09400.1720.1730.173 | ||||||
货期：3-5 working days
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