Mouse Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04717m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠可溶性CD40配体(sCD40L)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液.样本中的CD40LG含量。CD40LG是重要靶点。其背景在于它是免疫调节关键分子，参与多种免疫反应。研究机制上，主要聚焦其与受体CD40相互作用，激活信号通路，促进免疫细胞活化、增殖等，对于肿瘤免疫治疗、自身免疫病研究有重要意义。试剂盒检测范围为0.274 ng/ml-200 ng/ml.，灵敏度为0.274 ng/ml.。适用于基础科研中探索sCD40L在免疫调控、心血管疾病模型、炎症反应通路中的生物学功能，或用于体外药物筛选、基因编辑模型验证等实验中生物标志物的动态监测，为疾病机制研究和治疗策略开发提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Cd40lg; Cd40l; Tnfsf5; CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD antigen CD154
缩写：
CD40LG
Uniprot No.：
P27548
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates, cell culture supernates.
检测范围：
0.274 ng/ml-200 ng/ml.
灵敏度：
0.274 ng/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse sCD40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-96
1:2	Average %	92
	Range %	90-95
1:4	Average %	93
	Range %	90-99
1:8	Average %	95
	Range %	89-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
200	3.091	2.934	3.013	2.890
66.66	2.697	2.667	2.682	2.560
22.22	1.897	1.928	1.913	1.790
7.40	0.801	0.829	0.815	0.693
2.469	0.377	0.381	0.379	0.256
0.823	0.202	0.213	0.208	0.085
0.274	0.148	0.154	0.151	0.029
0	0.120	0.125	0.122