| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | URIM; L40mt; NLVCF; MRPL22; MRP-L22; MRP-L40 |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human MRPL40 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MRPL40抗体的模拟参考文献示例(非真实文献，供参考格式和方向)：
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1. **文献名称**: *MRPL40 regulates mitochondrial ribosome assembly and cellular respiration*
**作者**: Zhang Y et al.
**摘要**: 本研究通过CRISPR-Cas9敲除MRPL40基因，利用特异性抗体进行Western blot和免疫荧光分析，发现MRPL40缺失导致线粒体核糖体大亚基组装缺陷，进而抑制呼吸链复合体活性，表明其在能量代谢中的关键作用。
2. **文献名称**: *MRPL40 overexpression correlates with poor prognosis in hepatocellular carcinoma*
**作者**: Wang L et al.
**摘要**: 通过免疫组化(使用MRPL40抗体)分析肝癌组织样本，发现MRPL40在肿瘤组织中显著高表达，且与患者生存率负相关，提示其可作为潜在癌症标志物。
3. **文献名称**: *Development and validation of a polyclonal antibody against human MRPL40*
**作者**: Kim S et al.
**摘要**: 本研究报道了一种针对人MRPL40蛋白的多克隆抗体制备，通过ELISA、免疫印迹和免疫沉淀验证其高特异性和灵敏度，为线粒体蛋白功能研究提供工具。
4. **文献名称**: *MRPL40 deficiency disrupts mitochondrial translation in neuronal cells*
**作者**: Chen R et al.
**摘要**: 利用MRPL40抗体检测神经元细胞中的蛋白表达，发现其缺失导致线粒体mRNA翻译障碍，引发氧化应激和神经退行性表型，支持其在神经系统疾病中的病理意义。
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**注意**：以上文献为示例，实际文献需通过PubMed、Google Scholar等平台检索。建议使用关键词“MRPL40 antibody”、“mitochondrial ribosome protein”或结合具体研究领域(如癌症、代谢疾病)进行查询。
The MRPL40 antibody is a research tool designed to detect mitochondrial ribosomal protein L40 (MRPL40), a component of the large subunit of the mitochondrial ribosome. MRPL40 is encoded by nuclear DNA and plays a critical role in mitochondrial translation, a process essential for synthesizing proteins required for oxidative phosphorylation (OXPHOS). These proteins are integral to the electron transport chain, which generates ATP, the primary energy currency of the cell. MRPL40's function is closely tied to cellular metabolism, energy homeostasis, and mitochondrial integrity.
Antibodies targeting MRPL40 are commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study its expression, localization, and interactions. Researchers employ these antibodies to investigate mitochondrial dysfunction in diseases such as metabolic disorders, neurodegenerative conditions, and cancer, where altered mitochondrial protein synthesis or energy production is implicated. MRPL40 expression levels may also serve as a biomarker for mitochondrial activity in pathological states.
Commercial MRPL40 antibodies are typically raised in hosts like rabbits or mice, using immunogenic peptides specific to human MRPL40. Validation often includes testing on knockout cell lines or tissues to confirm specificity. Due to its role in fundamental cellular processes, MRPL40 remains a protein of interest in studies linking mitochondrial biology to disease mechanisms and therapeutic targets.
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