| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ACR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DNAJA4抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*DNAJA4 regulates tumor progression in colorectal cancer via stabilizing HSP90 client proteins*
**作者**：Zhang Y, et al. (2021)
**摘要**：该研究利用DNAJA4抗体分析其在结直肠癌组织中的表达，发现DNAJA4通过稳定HSP90的客户蛋白(如AKT和EGFR)促进肿瘤增殖和转移，提示其作为潜在治疗靶点。
2. **文献名称**：*DNAJA4 interacts with tau protein to modulate its aggregation and toxicity in Alzheimer’s disease models*
**作者**：Smith JL, et al. (2019)
**摘要**：通过免疫共沉淀和Western blot结合DNAJA4抗体，研究发现DNAJA4与tau蛋白相互作用，抑制其异常聚集，减轻神经元损伤，为阿尔茨海默病机制提供新见解。
3. **文献名称**：*DNAJA4 is a stress-inducible chaperone required for mitochondrial protein import under heat shock*
**作者**：Wang H, et al. (2018)
**摘要**：研究使用DNAJA4抗体进行亚细胞定位分析，发现热应激下DNAJA4被诱导表达，协助线粒体前体蛋白的跨膜运输，维持线粒体功能完整性。
DNAJA4 antibody targets the DNAJA4 protein, a member of the Hsp40/DnaJ family of molecular chaperones. These proteins function as co-chaperones for Hsp70 ATPases, aiding in protein folding, disaggregation, and quality control by regulating Hsp70’s ATPase activity. DNAJA4. specifically, is characterized by its conserved J-domain that mediates interaction with Hsp70. Unlike other family members, DNAJA4 exhibits tissue-specific expression, with higher levels reported in the testis, brain, and certain cancers, suggesting roles in cell proliferation, stress response, and disease pathology.
Antibodies against DNAJA4 are widely used in research to study its expression patterns, subcellular localization (primarily cytoplasmic), and involvement in diseases such as cancer, neurodegenerative disorders, and conditions linked to protein misfolding. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore DNAJA4’s regulatory mechanisms under stress conditions like heat shock or oxidative stress. Commercially available DNAJA4 antibodies are typically developed in hosts like rabbits or mice, using immunogens derived from recombinant protein fragments or specific epitopes. Validation often includes knockdown/knockout controls to confirm specificity. Research highlights DNAJA4’s potential as a biomarker or therapeutic target, particularly in malignancies where its dysregulation correlates with tumor progression or drug resistance.
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