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产品描述：鸡CD8分子(CD8)酶联免疫试剂盒竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD8含量。CD8是重要的免疫相关靶点。CD8阳性T细胞为细胞毒性T细胞，在机体免疫防御中发挥关键作用。其研究机制主要聚焦于增强CD8 T细胞的识别和杀伤能力，如通过免疫检查点抑制剂等，以更好地对抗肿瘤、病毒感染等疾病。试剂盒检测范围为3.125 U/mL-800 U/mL，适用于家禽免疫学基础研究，如感染性疾病模型中的T细胞功能分析、疫苗免疫效果评估或免疫调节机制探索等领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CD8
Uniprot No.：Q6QR64
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：3.125 U/mL-800 U/mL
灵敏度：3.125 U/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken CD8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 82-94 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 94-106 | |||||
回收率：
| The recovery of chicken CD8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 92-108 | ||||
| EDTA plasma (n=4) | 92 | 85-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| U/mlOD1OD2Average 8000.1910.2000.196 2000.3380.3440.341 500.6050.6340.620 12.51.1641.1451.155 3.1251.7921.6981.745 02.6362.5642.600 | ||||||
货期：3-5 working days
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