首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠CD81抗原(CD81)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD81含量。CD81 是重要的细胞表面分子，属于四次跨膜蛋白超家族成员。它参与细胞黏附、迁移及信号传导等生理过程。在研究机制上，其与多种病毒感染相关，可作为病毒受体，在肿瘤进展、免疫调节等方面的作用也备受关注，是潜在的治疗靶点。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于体外研究 CD81 在免疫应答、肿瘤微环境调控或病原体感染模型中的表达动态；支持科研人员高效开展动物模型样本的定量分析，为探索 CD81 相关分子机制提供可靠工具；尤其在 B 细胞活化、肝脏代谢调控等研究中具有重要价值本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cd81 ELISA Kit; Tapa1CD81 antigen ELISA Kit; 26 kDa cell surface protein TAPA-1 ELISA Kit; Target of the antiproliferative ELISA Kit 1 ELISA Kit; CD antigen CD81 ELISA Kit
缩写：CD81
Uniprot No.：P35762
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD81 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 85-96 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 95-99 | ||||||
回收率：
| The recovery of mouse CD81 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 89-98 | |||||
| EDTA plasma (n=4) | 104 | 100-113 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected30002.6922.6002.6462.49715002.0571.9381.9981.8497501.3761.4281.4021.2533750.9090.8540.8820.733187.50.5890.6090.5990.450940.3650.3710.3680.219470.2390.2470.2430.09400.1520.1450.149 | |||||||
货期：3-5 working days
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