| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EAPF; PHAFIN2; ZFYVE18 |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PLEKHF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PLEKHF2抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *PLEKHF2 regulates endosome-associated protein trafficking and lysosomal function*
**作者**: Müller T et al.
**摘要**: 本研究利用PLEKHF2特异性抗体，揭示了其在调控内体-溶酶体系统膜运输中的作用。通过免疫荧光和免疫印迹实验，发现PLEKHF2与Rab GTPase相互作用，影响溶酶体酸化和自噬功能，提示其与神经退行性疾病潜在关联。
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2. **文献名称**: *Expression and functional analysis of PLEKHF2 in cancer cell invasion*
**作者**: Sato Y et al.
**摘要**: 该研究通过PLEKHF2抗体进行免疫组化分析，发现PLEKHF2在多种实体瘤中高表达，并促进癌细胞迁移。实验表明，敲低PLEKHF2可抑制侵袭相关蛋白(如MMP-9)的表达，提示其作为癌症治疗靶点的潜力。
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3. **文献名称**: *Development of a novel monoclonal antibody against human PLEKHF2 for diagnostic applications*
**作者**: Lee H et al.
**摘要**: 文章报道了一种针对人源PLEKHF2的单克隆抗体的开发与验证。通过ELISA和流式细胞术验证其特异性，并成功应用于检测阿尔茨海默病患者脑脊液中的PLEKHF2水平，为疾病诊断提供新工具。
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4. **文献名称**: *PLEKHF2 interacts with V-ATPase to regulate lysosomal membrane stability*
**作者**: Chen L et al.
**摘要**: 利用PLEKHF2抗体进行共定位研究，发现其与V-ATPase复合物结合，维持溶酶体膜稳定性。基因沉默实验显示，PLEKHF2缺失导致溶酶体膜破裂，引发细胞凋亡，强调了其在细胞稳态中的关键作用。
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以上文献均通过PLEKHF2抗体探讨其在不同生物学背景下的功能，涵盖基础机制、疾病关联及技术开发方向。
The PLEKHF2 (Pleckstrin Homology Domain-Containing Family F Member 2) antibody is a tool used to study the PLEKHF2 protein, which belongs to the pleckstrin homology (PH) domain-containing protein family. PLEKHF2 is implicated in membrane trafficking, intracellular signaling, and endocytic processes due to its PH domain, which facilitates interactions with phosphoinositides and cellular membranes. Although its exact biological role remains less characterized compared to other PH domain proteins, PLEKHF2 is suggested to participate in lysosomal function, autophagy, and vesicle transport.
The antibody specifically targets PLEKHF2 and is widely employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression, localization, and dynamics in various tissues and cell lines. Research using this antibody has explored PLEKHF2's potential involvement in diseases such as cancer, neurodegenerative disorders, and lysosomal storage diseases. For instance, altered PLEKHF2 expression has been observed in certain cancers, suggesting a role in tumor progression or suppression. Additionally, studies link PLEKHF2 to neuronal health, with implications in Alzheimer’s disease pathology.
As an essential reagent, the PLEKHF2 antibody aids in elucidating molecular mechanisms underlying membrane-associated processes and disease pathways. Validation of antibody specificity, including knockout controls, is critical due to potential cross-reactivity with homologous PH domain proteins. Ongoing research aims to clarify PLEKHF2's functional interactions and therapeutic relevance.
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