Rabbit Polyclonal RAB3IP Antibody

The RAB3IP (RAB3A-interacting protein) antibody is a tool used to detect and study the RAB3IP protein, a key regulator of vesicular trafficking and exocytosis. RAB3IP, also known as Rabin8 or GRAB, belongs to the SEC2 family of guanine nucleotide exchange factors (GEFs) and specifically activates RAB8 and RAB3A, small GTPases involved in membrane trafficking. This protein plays a critical role in mediating secretory vesicle docking and fusion at the plasma membrane, particularly in neurons and endocrine cells, by facilitating the transition of RAB proteins from their GDP-bound inactive state to GTP-bound active state. Researchers utilize RAB3IP antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, localization, and interactions in cellular pathways. Dysregulation of RAB3IP has been implicated in neurodevelopmental disorders, cancer progression, and ciliogenesis defects, as it is essential for primary cilium formation and polarized secretion. The antibody’s specificity helps elucidate its phosphorylation-dependent regulatory mechanisms and its partnership with other trafficking components like the exocyst complex. Commercial RAB3IP antibodies are typically validated for cross-reactivity across human, mouse, and rat samples, aiding comparative studies in model organisms.