| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RITA; C12orf52 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human RITA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RITA1抗体的3篇参考文献示例(注：以下内容为虚构，仅作示例，实际文献需通过数据库检索确认)：
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1. **文献名称**：*RITA1 modulates Notch signaling by interacting with RBP-J and regulating transcriptional activity*
**作者**：Tanaka, N. et al.
**摘要**：本研究通过免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)实验，利用特异性RITA1抗体揭示了RITA1蛋白与RBP-J的相互作用机制，证明其在Notch信号通路中负向调控靶基因表达，影响细胞分化。
2. **文献名称**：*Development and validation of a monoclonal antibody for RITA1 in human cancer tissues*
**作者**：Smith, L. et al.
**摘要**：作者开发了一种高特异性RITA1单克隆抗体，并通过Western blot、免疫组化(IHC)和免疫荧光(IF)验证其在乳腺癌和肺癌组织中的表达，发现RITA1低表达与患者预后不良相关。
3. **文献名称**：*Subcellular localization and tubulin association of RITA1 revealed by antibody-based imaging*
**作者**：Garcia-Moreno, J. et al.
**摘要**：使用RITA1抗体进行共聚焦显微镜分析，发现RITA1定位于细胞质并与微管蛋白共定位，提示其在细胞骨架调控和有丝分裂中的潜在作用。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“RITA1 antibody”或“RBP-J interacting and tubulin-associated 1”获取最新研究。
RITA1 (RBP-J interacting and tubulin-associated 1), also known as C12orf52. is a protein encoded by the human gene *C12orf52*. It was initially identified as a binding partner of RBP-J (recombination signal-binding protein for immunoglobulin kappa J region), a key transcription factor in the Notch signaling pathway. RITA1 is implicated in regulating Notch signaling by modulating the interaction between RBP-J and its co-activators or co-repressors, thereby influencing transcriptional activity. Beyond its role in Notch signaling, RITA1 associates with microtubules and is involved in mitotic spindle organization, suggesting a dual function in transcriptional regulation and cell cycle progression.
Antibodies targeting RITA1 have been developed to explore its cellular localization, protein-protein interactions, and mechanistic roles in both physiological and pathological contexts. These antibodies are critical tools for studies using techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. Research has linked RITA1 to cancer biology, particularly in tumors where Notch signaling is dysregulated, such as T-cell acute lymphoblastic leukemia (T-ALL) and breast cancer. Its involvement in mitosis also positions it as a potential biomarker or therapeutic target in malignancies with chromosomal instability. The development and validation of RITA1 antibodies continue to support investigations into its multifaceted roles in cellular processes and disease mechanisms.
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