| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DDK1; MTDPS11; C20orf72; bA504H3.4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human MGME1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MGME1抗体的3篇代表性文献(信息简要概括)：
1. **文献名称**：*MGME1 processes flaps for mitochondrial DNA replication*
**作者**：Kornblum et al.
**摘要**：研究发现MGME1蛋白通过其外切酶活性参与线粒体DNA复制中的“flap”剪切，并通过特异性抗体验证了其在HEK细胞线粒体中的定位及功能缺陷导致的DNA复制异常。
2. **文献名称**：*Deficiency of MGME1 causes mtDNA depletion-like syndrome*
**作者**：Nicholls et al.
**摘要**：利用MGME1抗体检测患者细胞中的蛋白表达水平，发现MGME1缺失导致线粒体DNA耗竭综合征，揭示其与人类遗传病的直接关联。
3. **文献名称**：*Structural insights into MGME1 and its role in mitochondrial DNA maintenance*
**作者**：Xie et al.
**摘要**：通过免疫沉淀结合MGME1抗体，解析了MGME1与DNA复合物的结构，阐明其在DNA修复中的分子机制及与其他线粒体酶(如POLG)的相互作用。
(注：以上文献为虚拟示例，实际引用需查询具体数据库如PubMed获取真实文献。)
MGME1 (Mitochondrial Genome Maintenance Exonuclease 1) is a nuclear-encoded protein critical for mitochondrial DNA (mtDNA) replication and repair. It functions as a 5’-to-3’ exonuclease and flap endonuclease, resolving RNA/DNA hybrid primers and processing flap structures during mtDNA synthesis. MGME1 interacts with mitochondrial DNA polymerase γ and other replication machinery components, ensuring mtDNA integrity and accurate replication. Mutations in MGME1 are linked to mitochondrial disorders characterized by mtDNA deletions, depletion, or rearrangements, leading to multisystemic symptoms such as encephalopathy, myopathy, and respiratory failure.
Antibodies targeting MGME1 are essential tools for studying its expression, localization, and molecular interactions. They enable detection of MGME1 in tissues or cultured cells via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers use these antibodies to investigate MGME1’s role in mtDNA maintenance, its involvement in mitochondrial diseases, and potential therapeutic strategies. Commercial MGME1 antibodies are typically raised in rabbits or mice using immunogenic peptide sequences, with validation in knockdown/knockout models to confirm specificity. Such reagents have advanced understanding of mitochondrial genome dynamics and pathologies linked to mtDNA instability.
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