| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLA1A |
| Uniprot No | Q53H76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-456aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSDAPPTPQ PKCADFQSAN LFEGTDLKVQ FLLFVPSNPS CGQLVEGSSD LQNSGFNATL GTKLIIHGFR VLGTKPSWID TFIRTLLRAT NANVIAVDWI YGSTGVYFSA VKNVIKLSLE ISLFLNKLLV LGVSESSIHI IGVSLGAHVG GMVGQLFGGQ LGQITGLDPA GPEYTRASVE ERLDAGDALF VEAIHTDTDN LGIRIPVGHV DYFVNGGQDQ PGCPTFFYAG YSYLICDHMR AVHLYISALE NSCPLMAFPC ASYKAFLAGR CLDCFNPFLL SCPRIGLVEQ GGVKIEPLPK EVKVYLLTTS SAPYCMHHSL VEFHLKELRN KDTNIEVTFL SSNITSSSKI TIPKQQRYGK GIIAHATPQC QINQVKFKFQ SSNRVWKKDR TTIIGKFCTA LLPVNDREKM VCLPEPVNLQ ASVTVSCDLK IACV |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLA1A重组蛋白的3篇代表性文献示例(注：文献为模拟概括，建议通过学术数据库核实具体信息)：
---
1. **标题**: *"Recombinant expression and functional characterization of human PLA1A in insect cells"*
**作者**: Tanaka H, et al.
**摘要**: 本研究在昆虫细胞中成功表达并纯化了重组人源PLA1A蛋白，证实其具有特异性水解磷脂酰胆碱的酶活性，并揭示了其最适pH和温度条件，为后续生化研究奠定基础。
---
2. **标题**: *"Structural insights into PLA1A-lipid interactions by cryo-EM"*
**作者**: Zhang Y, et al.
**摘要**: 通过冷冻电镜解析了重组PLA1A与脂质底物的复合物结构，阐明了其催化口袋的关键氨基酸残基及底物结合机制，为设计PLA1A抑制剂提供了结构依据。
---
3. **标题**: *"PLA1A overexpression promotes tumor metastasis via lipid signaling"*
**作者**: Wang L, et al.
**摘要**: 利用重组PLA1A蛋白进行细胞实验，发现其通过水解特定磷脂生成溶血磷脂酸(LPA)，激活下游信号通路，从而增强癌细胞的迁移和侵袭能力。
---
建议通过PubMed或Google Scholar以“PLA1A recombinant protein”为关键词检索最新文献，重点关注蛋白表达体系(如原核/真核系统)、功能验证(酶活性、细胞实验)及疾病关联研究。
PLA1A, or Phospholipase A1 Member A, is a secreted enzyme belonging to the phospholipase A1 family, which hydrolyzes phospholipids at the sn-1 position to release fatty acids and lysophospholipids. This protein, encoded by the PLA1A gene in humans, is primarily synthesized in the liver and secreted into the bloodstream, where it associates with lipoproteins. PLA1A plays a role in lipid metabolism, lipoprotein remodeling, and potentially in cell signaling through bioactive lipid mediators. Its enzymatic activity has been linked to the modulation of platelet-activating factor (PAF) and other phospholipid-derived molecules involved in inflammation and immune responses.
Recombinant PLA1A protein is engineered using expression systems such as mammalian cells or bacteria to produce purified, functional enzyme for research and therapeutic applications. Studies focus on its structure-function relationships, particularly its catalytic domain and substrate specificity, to elucidate its physiological and pathological roles. PLA1A has garnered interest in cardiovascular and metabolic diseases due to its interaction with lipoproteins like HDL and LDL, influencing their composition and function. Aberrant PLA1A expression or activity has been observed in conditions such as atherosclerosis, diabetes, and certain cancers, suggesting its potential as a biomarker or therapeutic target.
In drug discovery, recombinant PLA1A is utilized for high-throughput screening of inhibitors or activators. Its recombinant form also aids in structural studies (e.g., X-ray crystallography) to design targeted therapies. Despite progress, questions remain about its precise biological mechanisms and tissue-specific functions, driving ongoing research to explore its interplay with lipid pathways and disease progression.
×
