| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | C16orf48; DAKV6410 |
| WB Predicted band size | 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ENKD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及ENKD1抗体的研究文献示例(注：以下文献信息为模拟创作，实际文献需通过学术数据库验证)：
1. **《ENKD1 regulates skeletal development through BMP signaling pathway》**
- 作者：Chen L, et al.
- 摘要：研究通过构建Enkd1基因敲除小鼠模型，发现ENKD1蛋白通过调控BMP信号通路参与成骨细胞分化。文中使用ENKD1多克隆抗体进行Western blot和免疫荧光实验，证实其在骨发育中的关键作用。
2. **《A novel antibody-based detection of ENKD1 in human osteoarthritis tissues》**
- 作者：Wang Y, et al.
- 摘要：开发了一种高特异性ENKD1单克隆抗体，用于检测骨关节炎患者软骨组织中ENKD1的表达水平。免疫组化结果显示ENKD1表达下调与关节退变程度显著相关。
3. **《ENKD1 interacts with RUNX2 to modulate osteoblastogenesis: Insights from antibody-mediated inhibition studies》**
- 作者：Kimura T, et al.
- 摘要：利用ENKD1中和抗体阻断其功能，发现ENKD1通过与转录因子RUNX2相互作用调控成骨细胞活性。研究为骨骼发育异常疾病提供了潜在治疗靶点。
(注：真实文献需通过PubMed或Web of Science等平台以"ENKD1 antibody"为关键词检索获取，以上内容为领域相关方向示例。)
The ENKD1 (Enkurin Domain Containing 1) antibody is a tool used to detect and study the ENKD1 protein, which is encoded by the ENKD1 gene. Enkurin, a conserved domain in ENKD1. is implicated in calcium signaling pathways, particularly through interactions with transient receptor potential (TRP) ion channels. ENKD1 is predominantly expressed in testicular tissues and plays a role in sperm development and function, suggesting its importance in male fertility. It has also been linked to cilia function and cellular mechanotransduction processes.
The ENKD1 antibody is widely utilized in research to investigate protein localization, expression levels, and molecular interactions via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Its applications extend to studying reproductive biology, ciliopathies, and conditions involving calcium signaling dysregulation. Commercial ENKD1 antibodies are typically raised in rabbits or mice, validated for specificity using knockout controls or siRNA silencing. Recent studies highlight ENKD1's potential role in cancer progression, particularly in tumors reliant on calcium-dependent signaling, though its mechanistic contributions remain under exploration. Researchers rely on this antibody to unravel ENKD1's physiological and pathological roles, bridging gaps in understanding cellular signaling and developmental disorders.
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