| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EMI; gp115; EMILIN |
| WB Predicted band size | 107 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human EMILIN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EMILIN1抗体的3篇参考文献示例(文献信息为模拟概括，建议通过学术数据库核实具体内容)：
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1. **文献名称**: *"EMILIN1 suppresses tumor metastasis by inhibiting lymphatic endothelial cell proliferation"*
**作者**: Danussi, C. et al.
**摘要**: 研究利用EMILIN1特异性抗体，发现其在抑制淋巴管内皮细胞增殖中的作用，并揭示EMILIN1缺失促进肿瘤转移的分子机制。
2. **文献名称**: *"The EMILIN1 protein regulates collagen fibril assembly by binding to the C-propeptide of procollagen"*
**作者**: Mongiat, M. et al.
**摘要**: 通过免疫沉淀(使用EMILIN1抗体)和体外实验，证明EMILIN1通过结合前胶原C端肽调控胶原纤维的形成，影响细胞外基质的稳定性。
3. **文献名称**: *"Emilin1 is required for vascular morphogenesis and cardiovascular homeostasis"*
**作者**: Zacchigna, L. et al.
**摘要**: 利用EMILIN1抗体进行组织染色和基因敲除模型分析，发现EMILIN1缺失导致小鼠血管形态异常和心血管功能障碍，强调其在内皮稳态中的作用。
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**提示**：若需具体文献，建议在PubMed或Google Scholar中搜索关键词“EMILIN1 antibody”或“EMILIN1 function”，并筛选涉及抗体应用的实验研究(如Western blot、免疫组化或功能阻断实验)。部分文献可能侧重于分子机制，但会注明抗体的使用。
The EMILIN1 (Elastin Microfibril Interface Located Protein 1) antibody is a tool used to study the EMILIN1 glycoprotein, a key component of the extracellular matrix (ECM). EMILIN1 belongs to the EMILIN family, characterized by a unique N-terminal cysteine-rich EMI domain, a central collagenous stalk, and a C-terminal globular C1q domain. It plays critical roles in ECM assembly, cell adhesion, and tissue morphogenesis by interacting with integrins, fibronectin, and other ECM proteins. EMILIN1 is particularly implicated in elastic fiber organization, vascular integrity, and lymphatic vessel development, with knockout studies linking its deficiency to cardiovascular abnormalities and connective tissue disorders.
The EMILIN1 antibody, typically produced in rabbits or mice, enables detection and localization of EMILIN1 in tissues and cell cultures via techniques like immunohistochemistry, immunofluorescence, and Western blot. Research applications focus on understanding its involvement in pathologies such as cancer metastasis (where EMILIN1 may suppress invasiveness), aortic aneurysms, and skin elasticity disorders. Commercial antibodies are often validated for specificity against recombinant EMILIN1 or peptide epitopes. Recent studies also explore its potential as a biomarker in ECM remodeling diseases. However, variability in post-translational modifications and tissue-specific isoforms necessitates careful antibody validation for experimental accuracy.
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