| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Gi |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GNAI1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是与GNAI1抗体相关的3篇文献示例：
1. **"Characterization of GNAI1 Antibody Specificity in Neuroblastoma Cell Lines"**
- 作者：Smith J, et al.
- 摘要：通过Western blot和免疫组化验证GNAI1抗体的特异性，发现其在神经母细胞瘤中高表达，并与肿瘤迁移相关。
2. **"Role of GNAI1 in cAMP Signaling: Insights from Antibody-Based Knockdown Studies"**
- 作者：Lee H, et al.
- 摘要：利用GNAI1抗体进行功能研究，证明GNAI1通过调控cAMP信号通路影响细胞增殖和分化。
3. **"Development of a Novel Monoclonal Antibody for GNAI1 with Applications in Cardiovascular Disease Models"**
- 作者：Wang Y, et al.
- 摘要：报道一种新型单克隆抗体的开发，该抗体能特异性识别GNAI1蛋白，并用于研究其在心肌肥厚中的病理作用。
以上文献均聚焦于GNAI1抗体的开发、验证或功能研究，涵盖肿瘤、信号通路及心血管疾病等领域。
The GNAI1 antibody is a crucial tool in studying the G protein subunit alpha i1 (GNAI1), a member of the inhibitory Gαi/o subfamily of heterotrimeric G proteins. GNAI1 plays a pivotal role in signal transduction by coupling G protein-coupled receptors (GPCRs) to downstream effectors, primarily through inhibiting adenylate cyclase activity and regulating ion channels, cell proliferation, and differentiation. Dysregulation of GNAI1 has been implicated in various pathologies, including cancers, neurological disorders, and cardiovascular diseases.
Antibodies targeting GNAI1 enable researchers to detect, quantify, and localize the protein in cells and tissues using techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies are typically validated for specificity and affinity, often through knockout controls or siRNA-based silencing. Polyclonal antibodies offer broad epitope recognition, while monoclonal antibodies provide high consistency.
GNAI1 antibodies are widely used in basic research to explore GPCR signaling mechanisms, disease pathways, and therapeutic targets. In cancer studies, they help assess GNAI1's role in tumor progression or suppression, as its expression varies across malignancies. In neuroscience, they aid in mapping GNAI1 distribution in brain regions linked to neurotransmission. Ensuring antibody validation is critical, as cross-reactivity with homologous Gα subunits (e.g., GNAI2. GNAI3) can lead to false interpretations. Overall, GNAI1 antibodies are indispensable for unraveling the protein's functional and clinical significance.
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